FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031966
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACAGAGACGTGTCCGTGGCGGTCACAGACCTCAGGCTTAGATAGAACTGATTTTTGGACGTGTCTACGGA29530.2861528383687059No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24880.2410932143113242No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19840.19225439597816207No Hit
CTGTATTACTGTGCGAGACAGTCCATCCGTCTCAACTGGAGCCACTGGAA19220.18624644610384453No Hit
CAGTAATACAGAGACGTGTCCGTGGCGGTCACAGACCTCAGGCTTAGATA18280.17713761887504045No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17900.17345532701658775No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17590.170451352079429No Hit
GTATTACTGTGCGAGACAGTCCATCCGTCTCAACTGGAGCCACTGGAACCCCTTTGACTCCTGGGGCCCGGGAGC17340.1680287916462364No Hit
GTTAGGACCCAGAGGGAACCATGGAAATCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA16640.16124562243329724No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16560.1604704030946756No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15750.15262130729113169No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15570.15087706377923304No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15130.14661335741681414No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14380.13934567611723642No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13840.13411294558154047No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA13690.13265940932162493No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13670.13246560448696953No Hit
ATCTAAGCCTGAGGTCTGTGACCGCCACGGACACGTCTCTGTATTACTGTGCGAGACAGTCCATCCGTCTCAA13390.12975233680179385No Hit
GTCTATTACTGTCAACACTTTGGTGGCTCGCTTACTTTCGGCGGAGGGAC12880.12481031351808103No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12770.1237443869274763No Hit
GTCTCTCGTGGGTCCATCAGCAGTCCTAATGACTACTGGGGCTGGGTCCG12190.11812404672246954No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT12170.11793024188781413No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCGCCTGTGGTT12010.11637980321057088No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11870.11502316936798307No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11660.1129882186041013No Hit
GTAATAGACTGCAAAATCTTCAGGTTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11640.1127944137694459No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11380.11027495091892561No Hit
GTCTCGCACAGTAATACAGAGACGTGTCCGTGGCGGTCACAGACCTCAGG11260.10911212191099318No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT11260.10911212191099318No Hit
CCCTATCACTCGCCTGCACAGTCTCTCGTGGGTCCATCAGCAGTCCTAATGACTACTGGGGCTGGGTCCGCCAGT11150.10804619532038845No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA11010.10668956147780062No Hit
CTATTATAGTGGCAGCACCTTCTACAATCCGTCCCTCAAGAGTCGCGTCA10960.10620504939116211No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG10500.10174753819408779No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGGT200.007346586351.7717637
GTATCAA22700.042.5053061
GGTATCA8700.038.2838131
TATCAAC26050.036.8733752
TCAACGC27300.035.049184
ATCAACG27350.034.9851073
AACGCAG28200.034.0538566
CAACGCA28150.033.9925045
AGAGTAC29550.032.27065711
CGCAGAG29950.032.178588
GCAGAGT30450.031.537569
CAGAGTA32350.029.57925810
ACGCAGA33150.028.9688917
AGTACGG29350.027.81565913
CAGCGCT6350.027.5443654
GAGTACG29650.027.53421812
TGCTTTA6850.026.54029814
TGTTAGA4600.026.18153270
AGCGCTG6900.025.8470885
GCTTTAT7000.025.48154815
Produced by FastQC (version 0.11.5)