FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868266
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAGTGCTACTACCTGCATATGAGCAG47380.25360414416362553No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAGTGCTACTACCTGCATATGAGCAGCAGTGATAAT40590.21726028306461712No Hit
CTCCTGCACTGCAAGCAACAGTGATGTTGGGAATTATAACCTTGTCTCCTGGTACCAACAGAACCCAGGCAAAGC39360.2106766381232651No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGCAAGCAACAGTGATGTT37560.20104203577006702No Hit
GTGCTACTACCTGCATATGAGCAGCAGTGATAATCAGCCTCGTCCTCAGC37550.20098851020143813No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35630.1907116010246935No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG32050.17154944745555503No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31420.1681773366319357No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC30780.1647517002396875No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27670.14810524839610634No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG27480.14708826259215765No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC25820.13820301819976386No Hit
GTCGATCACCATCTCCTGCACTGCAAGCAACAGTGATGTTGGGAATTATAACCTTGTCTCCTGGTACCAACAGAA25440.13616904659186646No Hit
TAGTAGCACTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA24530.13129821984663853No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC24390.13054886188583426No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG24380.13049533631720536No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG23830.12755143004261707No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23560.12610623968963736No Hit
GTGATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGTAGG23370.12508925388568867No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23090.12359053796408007No Hit
CCACAGTGCTACTACCTGCATATGAGCAGCAGTGATAATCAGCCTCGTCC22710.12155656635618269No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22080.11818445553256336No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21920.1173280464345013No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT21570.11545465153249056No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21400.11454471686579962No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21020.11251074525790224No Hit
GATTATCACTGCTGCTCATATGCAGGTAGTAGCACTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT20960.11218959184612898No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20170.1079610719244476No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC19510.10442838439494162No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19480.104267807689055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15100.041.300521
GTATCAA33650.038.499641
TCTCGTA1350.038.39964738
TCGTATG1300.037.24812740
TGGTATC5150.035.454882
TATCAAC37100.034.915752
CTACGCT1800.034.46620628
TCAACGC38950.032.9000364
ATCAACG39250.032.7372133
GTGGTAT5900.032.7031751
ACTACGC1900.032.6478227
CTCGTAT1600.032.4162439
CAACGCA40100.031.8706135
AACGCAG40800.031.4074046
ACGCAGA43850.029.2228537
AGAGTAC45700.028.33985711
CGCAGAG45400.028.2995178
CAGAGTA47250.027.3372910
GCAGAGT48650.026.4090049
CGTATGC1901.8189894E-1225.49926641
Produced by FastQC (version 0.11.5)