FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868266
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAGTGCTACTACCTGCATATGAGCAG43180.2311234053394966No Hit
CTCCTGCACTGCAAGCAACAGTGATGTTGGGAATTATAACCTTGTCTCCTGGTACCAACAGAACCCAGGCAAAGC39400.2108907403977806No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37710.2018449192995002No Hit
GTGCTACTACCTGCATATGAGCAGCAGTGATAATCAGCCTCGTCCTCAGC36340.19451191639734386No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAGTGCTACTACCTGCATATGAGCAGCAGTGATAAT36010.19274557263259087No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGCAAGCAACAGTGATGTT33170.17754431114198943No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG30890.16534048149460517No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29970.16041612918074835No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29880.15993439906308846No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC26990.1444655097293426No Hit
GTCGATCACCATCTCCTGCACTGCAAGCAACAGTGATGTTGGGAATTATAACCTTGTCTCCTGGTACCAACAGAA24100.12899662039559678No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG23820.12749790447398818No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23520.12589213741512184No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC23180.12407226808173998No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23110.12369758910133782No Hit
CCACAGTGCTACTACCTGCATATGAGCAGCAGTGATAATCAGCCTCGTCC22120.11839855780707885No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21820.1167927907482125No Hit
TAGTAGCACTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA21190.11342067992459318No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21060.11272484753241775No Hit
GTGATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGTAGG21020.11251074525790224No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT20640.11047677365000487No Hit
GATTATCACTGCTGCTCATATGCAGGTAGTAGCACTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT20420.10929921114016955No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20160.10790754635581871No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19840.1061947281596946No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC19610.1049636400812304No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19390.10378607757139507No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19200.10276909176744639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6800.042.0076451
GTATCAA36200.037.8384781
TGGTATC7900.036.116852
GGTATCA16400.035.675021
TATCAAC40800.033.280962
TCAACGC42100.032.24814
ATCAACG42150.032.1265833
CAACGCA42700.031.9568065
AACGCAG43950.030.9688766
AGAGTAC46000.030.03705611
ACGCAGA45800.029.7929957
CAGAGTA47550.029.12787410
CGCAGAG47800.028.5456628
GCAGAGT49900.027.6191129
AGTACGG45250.025.29451813
GAGTACG46100.024.75357612
TACTGAT9150.023.68756370
CGAACTA1403.8443068E-722.1387529
GTACGGG52750.021.763314
CTAGCGG800.002963944321.4817264
Produced by FastQC (version 0.11.5)