FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298144
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT8010.268662123000966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2550.051.07687470
CGCGGTT200.00858400749.76381326
TATACAC1750.043.4124343
CTTATAC1750.041.53191
TCGTATG1900.038.5719840
ATAACGT400.001816614537.0939959
CTCGTAT1850.035.62700339
CGCGTAG2000.035.00568828
ACGCGTA2000.034.9170227
GTATCAA15800.034.29131
GCGTAGT2050.034.27373529
TACACAT2250.033.7652245
CGTAGTA2100.033.55460730
GACGCGT2100.033.17587726
AGACGCG2100.033.12565225
GTAACGT400.003209679233.03119712
CGTATGC2250.032.70519641
AATCTCG1900.032.49732636
ATCTCCG2150.032.26302710
TGCCGTC2350.031.72557845
Produced by FastQC (version 0.11.5)