FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649606
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18860.29032983069737656No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA15600.24014556515795726No Hit
GTAATACACAGCCGTGTCCTCAGGTCGCAGGCTGTTCATTTGCAGATGCA14470.22275040563048987No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12390.19073099694276222No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11380.17518311099343292No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTATT10970.1688715929347943No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10830.16671644042696648No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10710.16486916684882838No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10630.16363765113006962No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10350.15932734611441396No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGTCTGGTACCAACAGAAACCTGGCCAGACTCCCAGGCT10310.1587115882550346No Hit
GTCTCCAGCCACCCTGTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG10260.15794189093081035No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT9880.15209219126670628No Hit
GTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGTCTGGTA9710.1494752203643439No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAACCT9580.14747400732136096No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9310.14331764177055015No Hit
TCTCTGATGCATCCAATAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT9280.1428558233760156No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCACGGAGGTTTC9270.14270188391117078No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9130.14054673140334295No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9060.13946915514942904No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9040.13916127621973934No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8550.13161824244234197No Hit
GTCCTGTGTAGCGTCTGGTTTCAACTTCAATTACTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG8470.13038672672358323No Hit
GTTTATTACTGTCAGCACGGAGGTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT8190.12607642170792757No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7880.12130429829773738No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7800.12007278257897863No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA7760.11945702471959926No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC7730.11899520632506473No Hit
CTCCCAGGCTCCTCATCTCTGATGCATCCAATAGGGCCACTGGCATCCCA7700.1185333879305302No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA7690.11837944846568535No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7600.11699399328208177No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTA7500.1154545986336333No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7230.11129823308282251No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7140.10991277789921892No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAGTAAT7120.10960489896952921No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7070.108835201645305No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7040.10837338325077046No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT6990.10760368592654625No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC6960.10714186753201171No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6910.10637217020778748No Hit
CATCAGAGATGAGGAGCCTGGGAGTCTGGCCAGGTTTCTGTTGGTACCAGACTAAGTTGCTGCTAACACTCTGA6790.10452489662964935No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6710.10329338091089059No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC200.00778549851.01533
GGTATCA5850.040.7398721
GTATCAA14150.038.9794851
TATCAAC16000.034.230672
ATCAACG16000.034.2227633
TCAACGC16000.034.0049634
CAACGCA16100.033.7963525
AACGCAG16500.033.1831556
TCTGGAG6700.032.81576570
AGAGTAC17500.031.28697611
CGCAGAG18300.030.1050728
GCAGAGT18250.030.001219
ACGCAGA19650.027.8637187
GAGTACG17000.027.806212
AGTACGG16800.027.52994713
TACGGGT2700.026.46052615
CAGAGTA21100.025.94891410
CGGAATT5250.025.80692760
ACATAGG801.0453896E-425.5351721
CTCTGGA6500.025.07046969
Produced by FastQC (version 0.11.5)