FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1276088
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC38960.3053080978741278No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37150.2911241231012281No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG32840.2573490229513952No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG32840.2573490229513952No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT32530.25491972340465546No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC31190.24441888020261923No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30880.24198958065587955No Hit
GTATAGGGGCCTGGCCTGGCCTCTGCTGGTACCAATAAGCATATTGCTTT30480.23885500059557022No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTCCGATAAGTGCCACTGCTGTCTG29850.23391803700058303No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTCCGATAAGTG29010.22733541887393344No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC28510.2234171937985468No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA28460.22302537129100813No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28380.22239845527894628No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC28290.22169317476537667No Hit
CTATTACTGTCAGTCAGCAGACAGCAGTGGCACTTATCGGAGGGTGTTCG28110.2202826137382375No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT27180.21299471509801832No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG26570.2082144805060466No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA26490.20758756449398472No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC26290.2060202744638301No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT26270.20586354546081465No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25790.20210204938844342No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25220.19763527280250265No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24860.19481415074822428No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24370.19097429017434533No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23950.18768298111102055No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC23780.1863507845853891No Hit
GTGTCAGTGTCCCCGGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC23480.1839998495401571No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23170.18157054999341737No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC23130.18125709198738646No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTCCCCGGGACAGACGG22300.1747528383622446No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG22240.17428265135319823No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21930.1718533518064585No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC21700.17005096827178062No Hit
CAGTAATAGTCACCCTCGTCCTCTGCCCGGACTCCACTGATGGTCAACGT21590.16918895875519557No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20830.16323325664060784No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20750.162606340628546No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20640.16174433111196093No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20590.16135250860442227No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTCCCCGGGACAGACGGCCAGGATCAC20320.15923666706371348No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19660.1540646099642031No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19590.15351605845364896No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA19470.15257568443555616No Hit
TCAGTGGAGTCCGGGCAGAGGACGAGGGTGACTATTACTGTCAGTCAGCA19390.1519487684234943No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19190.15038147839333965No Hit
CCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTCCCCGGGACAGACGGCCAGGATCACCTGCTCTGGAGATG19160.15014638488881643No Hit
GGACGAGGGTGACTATTACTGTCAGTCAGCAGACAGCAGTGGCACTTATC19110.14975456238127777No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC19060.1493627398737391No Hit
CCCCTATACTATTGATATATAAGGACACTGAGAGGCCCTCAGGGATCCCT18780.1471685338315226No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT18740.14685507582549168No Hit
GGCCAGGCCAGGCCCCTATACTATTGATATATAAGGACACTGAGAGGCCC18620.14591470180739885No Hit
GGCCTGGCCTCTGCTGGTACCAATAAGCATATTGCTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGCCGT17530.13737297114305597No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17490.13705951313702502No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG17370.13611913911893223No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG16710.13094708201942187No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC16640.13039853050886774No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16590.13000670800132907No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT16520.1294581564907749No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16480.129144698484744No Hit
GTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACC16400.12851778247268214No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA16090.1260884829259424No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15750.12342408987467948No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG15300.1198976873068315No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT15150.11872221978421552No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTG15130.11856549078120004No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14890.11668474274501445No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14830.11621455573596806No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14790.11590109772993712No Hit
GACTATTACTGTCAGTCAGCAGACAGCAGTGGCACTTATCGGAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTC14780.11582273322842938No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGT14740.11550927522239847No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG14670.11496072371184433No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTCCGATAAGTGC14520.11378525618922833No Hit
ATCGGAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCC14510.11370689168772058No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTC14280.11190450815304273No Hit
CCCTCGTCCTCTGCCCGGACTCCACTGATGGTCAACGTGACTGTTGTCCC14260.11174777915002726No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA14170.11104249863645767No Hit
GCAGTGGCACTTATCGGAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCT14000.10971030211082622No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC13930.10916175060027208No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG13910.10900502159725661No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13900.10892665709574888No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCG13810.10822137658217927No Hit
GTAATAGTCACCCTCGTCCTCTGCCCGGACTCCACTGATGGTCAACGTGA13780.10798628307765608No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA13580.10641899304750142No Hit
CCCCGGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAG13520.10594880603845504No Hit
CAGTCACGTTGACCATCAGTGGAGTCCGGGCAGAGGACGAGGGTGACTAT13510.10587044153694729No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13440.10532189002639317No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC13430.10524352552488545No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC13410.10508679652186997No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG13390.1049300675188545No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC13210.1035195064917153No Hit
GCAGACAGCAGTGGCACTTATCGGAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGG13100.10265749697513024No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG13040.10218730996608384No Hit
GCTTGGTCCCTCCGCCGAACACCCTCCGATAAGTGCCACTGCTGTCTGCTGACTGACAGTAATAGTCACCCTCGT12970.1016387584555297No Hit
CTGCTGGTACCAATAAGCATATTGCTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGCCGTCTGTCCCGGGGA12970.1016387584555297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2600.052.811611
GGTATCA10350.047.096731
CGTATGC900.046.051641
TGGTATC3000.045.7664762
TCGTATG851.8189894E-1244.6724840
TATCTCG803.45608E-1143.0494636
GTATCAA28150.043.046621
TATACAC1050.042.497443
CTTATAC955.456968E-1239.7476841
TCTCGTA901.2369128E-1038.30685438
TATCAAC32800.036.941082
ATGCCGT1250.036.0225244
GACCGGA1151.8189894E-1235.8256626
ATCAACG35250.034.7630423
AGATCGG400.002598720134.49381639
AACGCAG35600.034.4212726
GCCTATC1102.910383E-1134.3854933
TCAACGC35650.034.3743444
CAACGCA36300.033.853395
CGCAGAG37800.032.3258488
Produced by FastQC (version 0.11.5)