FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1276088
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC35890.2812501959112538No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT35560.27866416736149857No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35290.27654832582078975No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG32940.2581326679664725No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG30130.23611224304279954No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29240.2291378024086113No Hit
GTATAGGGGCCTGGCCTGGCCTCTGCTGGTACCAATAAGCATATTGCTTT28190.22090952975029934No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT28110.2202826137382375No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC27800.21785331419149775No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC27490.21542401464475805No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT27160.21283798609500285No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27090.21228943458444874No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTCCGATAAGTGCCACTGCTGTCTG26820.21017359304373995No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC26550.20805775150303116No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA26130.20476644243970632No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTCCGATAAGTG25810.2022587783914589No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24860.19481415074822428No Hit
CTATTACTGTCAGTCAGCAGACAGCAGTGGCACTTATCGGAGGGTGTTCG24840.1946574217452088No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24760.19403050573314692No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24440.19152284168489947No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG24220.18979882265172934No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24160.18932863564268296No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23550.18454840105071124No Hit
GTGTCAGTGTCCCCGGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC23230.1820407370024638No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC22590.17702540890596885No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC22420.17569321238033742No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTCCCCGGGACAGACGG21760.170521155280827No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21610.16934568775821104No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC21340.16722984621750225No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC21340.16722984621750225No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA21330.1671514817159945No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21280.16675965920845584No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG21200.16613274319639398No Hit
GGCCAGGCCAGGCCCCTATACTATTGATATATAAGGACACTGAGAGGCCC20890.16370344364965425No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA20660.1619010601149764No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20470.16041213458632947No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20320.15923666706371348No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTCCCCGGGACAGACGGCCAGGATCAC20050.1571208255230047No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG19520.15296750694309483No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC19350.15163531041746336No Hit
CAGTAATAGTCACCCTCGTCCTCTGCCCGGACTCCACTGATGGTCAACGT18990.148814188363185No Hit
CCCCTATACTATTGATATATAAGGACACTGAGAGGCCCTCAGGGATCCCT18790.1472468983330303No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18780.1471685338315226No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18200.14262339274407407No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18190.14254502824256635No Hit
CCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTCCCCGGGACAGACGGCCAGGATCACCTGCTCTGGAGATG18100.14183974772899674No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18040.14136956071995035No Hit
GGACGAGGGTGACTATTACTGTCAGTCAGCAGACAGCAGTGGCACTTATC17990.1409777382124117No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17970.14082100920939622No Hit
GGCCTGGCCTCTGCTGGTACCAATAAGCATATTGCTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGCCGT17960.14074264470788847No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT17950.14066428020638075No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17780.13933208368074929No Hit
GTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACC17540.13745133564456372No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT17020.13337638156616158No Hit
TCAGTGGAGTCCGGGCAGAGGACGAGGGTGACTATTACTGTCAGTCAGCA16510.1293797919892672No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16380.12836105346966667No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16030.12561829591689602No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15640.12256208035809442No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA15590.12217025785055576No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTG15550.12185679984452484No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15110.11840876177818457No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG15000.11754675226159951No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14710.11527418171787525No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG14590.11433380769978246No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC14470.11339343368168966No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGT14260.11174777915002726No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14210.1113559566424886No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC14200.11127759214098087No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTC14180.1111208631379654No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA14080.11033721812288806No Hit
GACTATTACTGTCAGTCAGCAGACAGCAGTGGCACTTATCGGAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTC13840.10845647008670249No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT13690.10728100256408649No Hit
ATCGGAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCC13550.10618389954297823No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT13450.1054002545279009No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13420.1051651610233777No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13410.10508679652186997No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13390.1049300675188545No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA13280.10406805800226944No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG13230.10367623549473078No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC13090.10257913247362252No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG13090.10257913247362252No Hit
CAGTCACGTTGACCATCAGTGGAGTCCGGGCAGAGGACGAGGGTGACTAT12930.10132530044949876No Hit
GCAGTGGCACTTATCGGAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCT12880.10093347794196011No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG12840.1006200199359292No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12840.1006200199359292No Hit
GTAATAGTCACCCTCGTCCTCTGCCCGGACTCCACTGATGGTCAACGTGA12800.10030656192989826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC3300.054.0224382
GTGGTAT3650.049.8166351
GGTATCA12400.046.4813731
GTATCAA30600.039.577161
TATCAAC33950.035.545752
GTAGCGC501.9361416E-434.2700353
ATCAACG36550.033.1917723
TCAACGC36550.033.0993084
AACGCAG37100.032.7009936
CAACGCA37350.032.3903545
CGCAGAG38550.031.3808638
ACGGGAC1001.5435944E-830.85149616
CAGAGTA39350.030.65458710
GCAGAGT39550.030.5874149
TAGACGT450.00477569230.4634484
AGAGTAC39400.030.44769911
TACGGGA6250.029.61743515
TGCGATT500.007975019527.42785318
CGCGCGT500.00799028827.4171037
ATAGACG500.00799181527.4160273
Produced by FastQC (version 0.11.5)