FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662941
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCTTCCCCTCTCAGGCTGTTCATTTGCAGATACA9830.14827865526494816No Hit
GTATATTACTGTGCGAGAAAGGGGTACAACCATGGTTACGATCGGGGCAATTGGGCAGAGTGGGACTACTTTGA6850.10332744542877874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13100.041.9384651
TATCAAC15650.034.885632
GTGACGT400.002660412834.326766
ATCAACG16150.034.015633
TCAACGC16500.033.2865524
CAACGCA16650.033.192845
AACGCAG17100.032.5200846
AGAGTAC18250.030.84240711
ATACGAA450.004653187530.62356237
TGTATAA1501.8189894E-1229.74536914
ACGCAGA19000.029.268087
CGCAGAG19250.029.2402048
GTGTATA1603.6379788E-1227.88628413
CAGAGTA20700.027.68938810
TTGAACG500.00770275727.62185340
TAATTGC500.00792488827.4614054
AGTACGG18350.027.12059413
GTATAAG1653.6379788E-1227.04328515
GTGGTAT2200.026.5332281
GAGTACG19050.026.12403912
Produced by FastQC (version 0.11.5)