FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662941
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9300.1402839770054952No Hit
GTAATATACAGCCGTGTCTTCCCCTCTCAGGCTGTTCATTTGCAGATACA8290.12504883541672637No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT6630.10000889973617562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14500.044.4794581
TATCAAC16700.039.007182
TCAACGC17650.036.7023164
ATCAACG18100.035.7871253
GTGGTAT2400.035.735381
AACGCAG18550.035.475926
CAACGCA18500.035.386535
AGAGTAC19900.032.89950611
CGCAGAG20300.032.248818
ACGCAGA20650.031.7022177
AGTCGAT450.00461868630.67017739
ATAGTAC450.0047734130.464293
TTCGGGG3450.030.31186970
CAGAGTA22200.029.48877510
AGTACGG20850.029.09888313
GAGTACG21450.028.2849312
CGTGTCT3050.028.0963113
GCAGAGT24000.027.2771179
TACGGGA8150.026.9213615
AATATAC3200.026.7752573
Produced by FastQC (version 0.11.5)