FastQCFastQC Report
Wed 8 May 2024
EGAF00005761216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773754
Total Bases57.5 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA18960.2450391209609256No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT18210.23534611775835731No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16730.2162185914386226No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT15280.19747878524699064No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15020.194118544136767No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14920.19282614370975787No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14800.19127526319734697No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13890.17951441931156414No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA13630.17615417820134047No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13510.17460329768892957No Hit
ATATTACTGTGCGAGGGGGAAATATTGTGGTGGGATCACCTGCAATGAGT13420.17344013730462138No Hit
CAGTAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA13150.16995065615169677No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13110.16943369598089317No Hit
GTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC13030.16839977563928588No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12830.1658149747852677No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12570.162454733675044No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12510.16167929341883855No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12390.16012841290642763No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12170.1572851319670076No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11950.1544418510275876No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA11380.1470751685936357No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11350.14668744846553297No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11190.14461960778231842No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11100.14345644739801022No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAG11070.1430687272699075No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT11000.14216404697100113No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10960.14164708680019747No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10950.14151784675749657No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCT10950.14151784675749657No Hit
CTATATTACTGTGCGAGGGGGAAATATTGTGGTGGGATCACCTGCAATGA10790.13945000607428198No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10110.1306616831706201No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9910.12807688231660191No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCC9860.12743068210309738No Hit
CTATAAAGCTGTTGACAGGAATAAGTTGCAAAATCTTCAGGCTGCAGGCT9790.126526001804191No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9630.12445816112097642No Hit
GCTTTATAGTTTTCCTCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCA9560.12355348082207006No Hit
GATCTATGCTGCATCCACTTTACAAAATGGGGTCCCATCAAGGTTCAGCG9390.12135640009615459No Hit
ATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCA9350.12083943992535096No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9340.12071019988265004No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAT9220.11915931937023913No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9060.11709147868702455No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9060.11709147868702455No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9030.11670375855892183No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8930.11541135813191272No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8920.11528211808921182No Hit
CTCCTGATCTATGCTGCATCCACTTTACAAAATGGGGTCCCATCAAGGTT8820.11398971766220271No Hit
GCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC8760.11321427740599727No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC8750.11308503736329634No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG8740.11295579732059544No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC8710.11256807719249269No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCATAGTTGT8680.11218035706438997No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8670.11205111702168907No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCC8480.10959555621037177No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8420.10882011595416631No Hit
GGGCTGGAGTGGGTCTCAGGTATTAGTGGTAGTGGTGGTAGCAAATCCTT8340.10778619561255902No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC8270.10688151531365266No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA8150.10533063480124175No Hit
CTCCTGTGAAGCCTCTGGATTCAGCTTTAACAACTATGCCATGAGCTGGG8130.10507215471583992No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC8070.10429671445963445No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG7940.10261659390452263No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT7930.10248735386182173No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG7880.10184115364831715No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC7860.10158267356291534No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC7820.1010657133921117No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT7790.10067799326400897No Hit
ATGCTGCATCCACTTTACAAAATGGGGTCCCATCAAGGTTCAGCGGCAGT7780.10054875322130807No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTA7750.10016103309320533No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT7750.10016103309320533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)