FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835684
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23250.27821521053412535No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22490.26912086386720335No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14860.1778184098295528No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14690.17578414807510973No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA14370.171954949478511No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13900.16633081403975664No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12990.15544153053067908No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11800.1412016982495776No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11570.13844946175827225No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11520.13785114947755372No Hit
CCATTATATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC11370.13605621263539808No Hit
GTAATATATGGCCGTGTCTGCGGCGGTCACAGAGCTCAACTTCAGGGAGA11340.13569722526696693No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG10970.13126971438964968No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATCTTTATTACTGGTTGGCCTGGTATCAGCAGAAA10930.13079106456507483No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10880.13019275228435628No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAATAA10870.13007308982821258No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC10850.12983376491592516No Hit
ATATAATGGTTATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTT10500.12564557895089531No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10460.12516692912632046No Hit
CTTTATTACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA10340.12373097965259595No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA10140.12133773052972177No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9720.11631190737168594No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9630.11523494526639257No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9510.11379899579266806No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9290.11116642175750643No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9260.1108074343890753No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA9150.1094911473714945No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8570.10255072491515932No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8560.10243106245901562No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8460.10123443789757851No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22750.042.0641141
TATCAAC24050.039.4649472
GTGGTAT2900.037.8478241
TCAACGC25050.037.6136784
CAACGCA25100.037.5387545
ATCAACG25350.037.16413
AACGCAG25750.036.591176
AGAGTAC27450.034.32916311
CGCAGAG27550.034.3248258
ACGCAGA27750.034.200917
CAGAGTA28100.033.8968510
GCAGAGT28900.032.6028639
GAGTACG24950.031.4512412
TACGGGT2400.031.41494415
AGTACGG25000.031.39021713
CGTCCTA450.004765342530.47574618
GTACGAA450.0047765230.46115113
TGCGACT450.00477791930.45932612
GTACGGG27500.028.03810714
TACGGGG12300.027.86247815
Produced by FastQC (version 0.11.5)