FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1601636
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19800.041.8825531
GTATCAA49850.039.543161
TATCAAC57100.034.576482
TCAACGC58400.033.6862144
ATCAACG58450.033.6003153
CAACGCA59900.032.8426555
AACGCAG61450.032.1218156
ACGCAGA65250.030.2511187
CGCAGAG67350.029.358328
AGAGTAC67800.029.11335411
CAGAGTA70250.028.19473610
GCAGAGT71850.027.566889
GTGGTAT6550.027.500661
AGTACGG57000.026.58311313
GAGTACG58950.025.64614312
ACGGGTA4650.024.84503216
TCGTATG3250.024.42046740
TACGGGT16050.024.34497315
GTACGGG62750.023.93213514
CTCGTAT3650.023.59540239
Produced by FastQC (version 0.11.5)