FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1221907
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG62340.5101861270947788No Hit
GATTATTACTGTGCAGCGTGGGATGACGGCCCCAGTGGTCCGGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTC54920.4494613747200073No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC45440.3718777288287897No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCAC42990.3518271030446671No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACCGGACCACTG40870.33447717379473235No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC40820.3340679773501584No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38130.3120532086320808No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC35710.2922481007147025No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG35270.2886471720024519No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33080.27072436773011366No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT32770.2681873497737553No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC31140.2548475456806451No Hit
CTTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC30100.2463362596335073No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG29220.2391344022090061No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCAC28900.23651554496373295No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG27480.22489436593783324No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27040.22129343722558262No Hit
CCCCTGAGGACCGCTGATTATCACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACTTGCTGATACCACTTTA26630.21793802638007642No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26350.21564652629046235No Hit
CTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACTTGCTGATACCACTTTACAGAATTACCTCCGATGTTGGAG25770.21089984753340477No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25480.20852650815487592No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25440.2081991509992168No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG25370.20762627597681332No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25130.2056621330428584No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24060.1969053291289763No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCGTGGGATGACGGCCCCA24010.19649613268440236No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22860.18708461445920188No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCTTCACTCA22710.1858570251254801No Hit
CCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT22490.18405656076935478No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22280.18233793570214427No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22220.18184689996865555No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT22000.18004643561253025No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGCCAGGGACC21880.17906436414555282No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21740.1779186141007458No Hit
GCGTGGGATGACGGCCCCAGTGGTCCGGTGGTTTTCGGCGGAGGGACCAA20970.17161698885430726No Hit
GTCCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAG20840.17055307809841502No Hit
TTACTGTGCAGCGTGGGATGACGGCCCCAGTGGTCCGGTGGTTTTCGGCG19400.15876822049468575No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCTTCA19210.15721327400530483No Hit
GCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAG19150.1567222382718161No Hit
CATCTATAGTGATAATCAGCGGTCCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC19080.15614936324941261No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18940.1550036132046056No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCTTCACTCACTGTGCAGGGTCCTGGGCCCAGT18760.15353050600413942No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18630.1524665952482472No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18610.15230291667041762No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGCC18040.14763807720227481No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17920.14665600573529738No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGG17890.146410487868553No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17790.14559209497940515No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACCGGACCACTGG17580.14387346991219463No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17370.1421548448449841No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17220.1409272555112623No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17040.13945414831079617No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCTTCACTCACTGTGCAGGGTCCTGGGC16820.13765368395467084No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16700.1366716124876934No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC16640.1361805767542047No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCT16430.13446195168699418No Hit
GTTTGGGGGCCGTTCCTGGGACTTGCTGATACCACTTTACAGAATTACCT16430.13446195168699418No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16370.13397091595350547No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG16170.13233413017520973No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16000.13094286226365837No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15790.12922423719644785No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCTTCACTCACTGTGCAGGGT15780.12914239790753307No Hit
CCTGAGGACCGCTGATTATCACTATAGATGAGGAGTTTGGGGGCCGTTCC15560.12734193355140774No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG15340.12554146919528245No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15080.12341364768349802No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC15070.12333180839458323No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15010.1228407726610945No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGCCAGGGACCCCTGAGGACCGCTG14790.1210403083049692No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14680.12014007612690654No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAGGTAATTCTGTAAAGTGGTATCAGCAAGTCCCAGGAACGGCCCC14460.11833961177078124No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14330.11727570101488902No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCTTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG14210.11629362954791159No Hit
GTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTGATAATCAGCGGTCCTCAGGGGTCCCTGGCCGATTCT14200.11621179025899679No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACCGGACCA14140.11572075452550808No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13990.11449316519178629No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGGAAGCAG13950.11416580803612712No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC13930.11400212945829756No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13760.1126108615467462No Hit
GTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGGAAGCAGCT13370.10941912927906951No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG13360.10933728999015474No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA13280.10868257567883645No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG13180.10786418278968858No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT13070.10696395061162592No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC13000.10639107558922242No Hit
GATTATCACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACTTGCTGA12910.10565452198898935No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGCGG12790.10467245052201189No Hit
GACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC12730.10418141478852319No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12570.1028719861658866No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT12530.10254462901022746No Hit
GCTTGGTCCCTCCGCCGAAAACCACCGGACCACTGGGGCCGTCATCCCACGCTGCACAGTAATAATCAGCCTCAT12450.10188991469890916No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12360.10115336109867608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22700.041.496231
TCGTATG1800.036.39755240
TATCAAC25850.036.3056372
ATCAACG27500.034.2518543
AGGGGGG3550.034.2230270
TCAACGC27600.034.1263584
CGTATGC1950.033.62822341
CAACGCA28150.033.4595875
AACGCAG28800.032.7004056
ACGCAGA30500.030.8777627
CGCAGAG31250.030.3558738
AGAGTAC31150.030.23344411
CTTATAC2400.029.9715961
ATGCCGT2200.029.905544
TATACAC2550.029.5506173
CAGAGTA32000.029.43036710
TTATACA2350.029.1505132
GCAGAGT33050.028.7026029
TACGGGT2050.028.39932615
GCCAGAG34600.028.28564670
Produced by FastQC (version 0.11.5)