FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1221907
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCGTGGGATGACGGCCCCAGTGGTCCGGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTC50470.4130428911529273No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC46360.3794069434089501No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC43050.3523181387781558No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36340.29740397591633405No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT35070.2870103862241562No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCAC32050.26229492097189067No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT31770.2600034208822767No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACCGGACCACTG31520.257957438659407No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCAC31330.25640249217002603No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG31320.2563206528811112No Hit
CTTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC30430.24903695616769525No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC29600.242244295187768No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC28840.2360245092302442No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG28030.2293955268281465No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG27230.2228483837149636No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26930.22039320504752No Hit
CTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACTTGCTGATACCACTTTACAGAATTACCTCCGATGTTGGAG26670.21826538353573555No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG26500.21687411562418416No Hit
CCCCTGAGGACCGCTGATTATCACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACTTGCTGATACCACTTTA26230.2146644548234849No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG25900.21196375828929698No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25020.2047619008647958No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24470.2002607399744825No Hit
CCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT24050.19682348984006148No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCGTGGGATGACGGCCCCA23960.19608693623982842No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23680.19379543615021438No Hit
GTCCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAG23160.18953979312664548No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22610.18503863223633224No Hit
CATCTATAGTGATAATCAGCGGTCCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC22230.18192873925757033No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCTTCACTCA21950.17963723916795632No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21840.17873700698989367No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21700.17759125694508665No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21570.17652734618919444No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG21110.17276273889911425No Hit
GCGTGGGATGACGGCCCCAGTGGTCCGGTGGTTTTCGGCGGAGGGACCAA20470.16752502440856792No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19770.16179627418453285No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19150.1567222382718161No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGG19060.15598568467158302No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCTTCA18950.15508545249352038No Hit
GTTTGGGGGCCGTTCCTGGGACTTGCTGATACCACTTTACAGAATTACCT18830.15410338102654295No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAGGTAATTCTGTAAAGTGGTATCAGCAAGTCCCAGGAACGGCCCC18730.15328498813739508No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18660.15271211311499155No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18270.1495203808473149No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCTTCACTCACTGTGCAGGGTCCTGGGCCCAGT17780.14551025569049034No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17720.14501921995700165No Hit
TTACTGTGCAGCGTGGGATGACGGCCCCAGTGGTCCGGTGGTTTTCGGCG17610.14411898777893897No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17170.14051805906668838No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGCC17160.1404362197777736No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17050.13953598759971095No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16960.13879943399947786No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG16550.1354440231539716No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG16140.13208861230846536No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16120.1319249337306358No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15950.13053366581908443No Hit
GTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTGATAATCAGCGGTCCTCAGGGGTCCCTGGCCGATTCT15940.13045182653016962No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15860.12979711221885137No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15740.12881504075187392No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGGAAGCAG15740.12881504075187392No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15620.12783296928489649No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15460.12652354066225988No Hit
CCTGAGGACCGCTGATTATCACTATAGATGAGGAGTTTGGGGGCCGTTCC15450.12644170137334512No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15360.125705147773112No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15070.12333180839458323No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCT14950.12234973692760578No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCTTCACTCACTGTGCAGGGTCCTGGGC14890.12185870119411707No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC14750.12071295114931006No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCTTCACTCACTGTGCAGGGT14540.11899432608209953No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC14080.11522971879201936No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGCCAGGGACCCCTGAGGACCGCTG14030.11482052234744543No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13920.11392029016938279No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13760.1126108615467462No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13490.11040120074604697No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13360.10933728999015474No Hit
GTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGGAAGCAGCT13220.10819153994534772No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACCGGACCA13190.10794602207860338No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGCGG13160.107700504211859No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCAC13150.10761866492294421No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA13120.10737314705619985No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACCGGACCACTGG13090.10712762918945548No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13030.10663659345596678No Hit
GAATTACCTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCG12890.10549084341115976No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT12820.10491796838875626No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCTTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG12640.1034448611882901No Hit
GATTATCACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACTTGCTGA12630.10336302189937532No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12560.10279014687697183No Hit
TTCCTGGGACTTGCTGATACCACTTTACAGAATTACCTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGA12510.1023809504323979No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAC12500.1022991111434831No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)