FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911948
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT10110.11086158421313495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1900.047.12698739
TCGTATG2200.040.78726640
GGTATCA15400.037.715421
GTATCAA34500.036.90061
CGTATGC2600.035.90611341
TCTCGTA2550.035.03779638
GTGGTAT5450.034.0782551
AGGGGGG4300.033.22715870
TATACAC2850.033.1723863
TATCAAC40150.032.4612542
ATCAACG41250.031.5137673
TCAACGC41200.031.470064
CAACGCA41550.031.2049675
AACGCAG41750.031.0520766
CTTATAC2950.029.7620261
TAGGCAT3100.029.5067129
ACGCAGA44400.029.2747847
TTATACA3000.029.2627852
TACACAT3350.029.2291875
AGAGTAC46050.028.07922211
Produced by FastQC (version 0.11.5)