FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911948
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23070.2529749503261151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36100.038.075691
GGTATCA15400.036.18461
GTGGTAT6150.034.596011
ATTATCG400.002807587433.95315630
TATCAAC41600.032.682812
ATCAACG42550.031.7875613
TCAACGC43050.031.3434544
AACGCAG43500.031.174316
CAACGCA43700.030.8772495
ACGCAGA44850.030.1590847
TGGTATC7000.029.3994672
CAGAGTA46300.029.0656810
AGAGTAC46500.028.86813411
CGCAGAG47350.028.5651688
GCAGAGT47350.028.3499099
AGTACGG43500.026.285913
GAGTACG44850.025.49328812
ACGGGTA2650.024.18753216
GTACGGG46900.024.0207214
TACGGGT8050.023.46542415
Produced by FastQC (version 0.11.5)