FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1733293
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAAGGTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGGTGATTGTGAGAGTGAATTCTGTC66610.3842974038434356No Hit
CCTTAATAGTTACCCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT56650.32683452826498466No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48490.2797565097187838No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34690.2001392724715325No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31470.18156191711384054No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30520.1760810203468196No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG30130.17383096798983208No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29160.1682346839224528No Hit
GATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA28950.1670231172686903No Hit
GTCTCCACCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCA28120.16223454430381937No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCACTTATTTAGCCTGGTATCAGCAGAAA27940.16119605860059436No Hit
GTAATACACGGCCGTGTCAGCGGCGGTCACAGACCTCAGTTTCAGGGAGA27830.1605614284486235No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC27550.15894600624360683No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27040.15600363008446927No Hit
GGGTAACTATTAAGGTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTG26870.15502283803142342No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG26450.1525997047238984No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT26410.15236893012318173No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT26360.15208046187228588No Hit
CCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC25430.1467149524056233No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23850.1375993556773148No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG23300.13442620491746057No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22860.1318876843095772No Hit
GGCCAAAAGTGTACATGGGGTAACTATTAAGGTGTTGACAGTAATAAGTT21800.12577215739058542No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21080.12161821457768536No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21050.12144513362714786No Hit
TAGTTACCCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT20850.1202912606235645No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA20610.11890661301926447No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20510.11832967651747282No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCACCCTTC20340.11734888446442696No Hit
GTGGATGCACCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAAATAAGTGCTAATG20240.11677194796263528No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20230.1167142543124561No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC20120.11607962416048526No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20060.11573346225941027No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19910.11486805750672276No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19800.1142334273547519No Hit
GTCTCTGGTGACTCCATCAGTAGTGACTACTGGAGCTGGATCCGGCAGCC19370.1117526003970477No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19180.11065642104364352No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18880.10892561153826848No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18840.10869483693755182No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18820.10857944963719349No Hit
GTGTATTACTGTGCGAGACTTAGTCGCGTCGTCCATAACAGTTTTCATTA18660.1076563512343268No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGCC18460.10650247823074345No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18110.10448320047447257No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18010.10390626397268089No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGGTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG17700.10211776081712672No Hit
GTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGA17670.10194467986658921No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT17480.10084850051318502No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)