FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863176
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGGTCCTTCAGTGGTGACTACTGGAGCTGGATCCGCCAGTC30010.347669536687767No Hit
GTATAATGACTGGCCTCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCACACGAACTGTGGCTGCACCAT26030.30156074775016917No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21270.24641556299062994No Hit
GTAATAAACAGCCGTATCAGCGGCGGTCACAGAGGTCAGGTTCAGGGAGA21170.2452570507057657No Hit
GTATTAGCACTTTCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCATCCA20740.24027544788084934No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCA19950.23112320083042162No Hit
GTCATTATACTGTTGACAGTAATAAATTGCAAAATCTTCAGACTGCAGGCTGCGGATGGTGAGAGTGAACT19280.223361168521831No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14650.16972204973261537No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14110.16346608339434832No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13990.1620758686525112No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT13570.15721011705608126No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13410.15535649740029842No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13310.15419798511543417No Hit
GTTTATTACTGTGCGAGAGGACTTATTGTGGTGGTCGCTGCTCCCTTCTT12830.14863712614808566No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12640.14643595280684357No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12400.14365552332316933No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC12240.1418019036673865No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12200.14133849875344076No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11590.13427157381576876No Hit
TTCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCATCCACCAGGGCCA11370.13172284678906737No Hit
GTCTGGGACAGAGTTCACTCTCACCATCCGCAGCCTGCAGTCTGAAGATT11170.1294058222193388No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10640.1232657071095582No Hit
CTCCTGCAGGGCCAGTCACAGTATTAGCACTTTCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT10620.12303400465258534No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA10490.12152793868226179No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC10360.12002187271193823No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10350.11990602148345181No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10340.11979017025496538No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10310.11944261656950611No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT10210.11828410428464183No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10010.1159670797149133No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9930.11504026988702189No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9890.11457686497307618No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCACAGTATTAGCA9780.11330250145972548No Hit
GAGTTCACTCTCACCATCCGCAGCCTGCAGTCTGAAGATTTTGCAATTTATTACTGTCAACAGTATAATGACTGG9770.11318665023123906No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT9770.11318665023123906No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9520.11029036951907838No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9430.10924770846270054No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9230.10693068389297201No Hit
GTGCAGCCACAGTTCGTGTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC9190.10646727897902629No Hit
GTGCTAATACTGTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACACAGACAGGGTGGCTGGAGA9120.10565632037962132No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8960.10380270072383847No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG8940.10357099826686562No Hit
CATACAACCCGTCCCTCAAGAGTCGCGTTAGCATATCATTAGACACGTCCAAGAACCAGTTCTCCCTGAACCTGA8820.10218078352502849No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCACAGTATTAGCACTTTCTTAGCCTGGTA8700.10079056878319137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2250.044.27261
GGTATCA7450.041.0348931
GTATCAA17050.039.6881941
CGTATGC702.1187589E-839.57287241
TCGTATG702.138404E-839.53131540
TAATGAT6800.038.39365470
TGGATTG11400.036.06982870
TATCAAC19100.035.2465862
ATCAACG19600.034.347443
TCAACGC19600.034.347444
CAACGCA20150.033.4099165
AACGCAG20650.032.6028486
TATACAC1305.456968E-1231.705333
AGAGTAC22150.030.39321911
CGCAGAG22450.029.9870748
TTATACA1302.0736479E-1029.063222
GCAGAGT23500.028.6472269
TCGTATA500.007044289728.1331261
ACGCAGA24000.028.0520347
CAGAGTA24350.027.6472210
Produced by FastQC (version 0.11.5)