FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863176
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGGTCCTTCAGTGGTGACTACTGGAGCTGGATCCGCCAGTC30630.35485231285392554No Hit
GTATAATGACTGGCCTCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCACACGAACTGTGGCTGCACCAT25590.2964632936967664No Hit
GTAATAAACAGCCGTATCAGCGGCGGTCACAGAGGTCAGGTTCAGGGAGA21020.24351928227846928No Hit
GTATTAGCACTTTCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCATCCA20930.24247662122209146No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19700.22822692011826093No Hit
GTCATTATACTGTTGACAGTAATAAATTGCAAAATCTTCAGACTGCAGGCTGCGGATGGTGAGAGTGAACT18730.21698935095507751No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCA18540.2147881776138354No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14040.16265512479494332No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13420.15547234862878487No Hit
GTTTATTACTGTGCGAGAGGACTTATTGTGGTGGTCGCTGCTCCCTTCTT13000.15060659703235493No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12900.14944808474749066No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12490.14469818437954715No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT12470.1444664819225743No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12020.1392531766406851No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11730.1358934910145787No Hit
GTCTGGGACAGAGTTCACTCTCACCATCCGCAGCCTGCAGTCTGAAGATT11490.13311306153090446No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11350.1314911443320945No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC11020.1276680537920424No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10930.12662539273566456No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10540.12210719482469393No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10480.12141208745377535No Hit
GAGTTCACTCTCACCATCCGCAGCCTGCAGTCTGAAGATTTTGCAATTTATTACTGTCAACAGTATAATGACTGG10350.11990602148345181No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10350.11990602148345181No Hit
GTGCTAATACTGTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACACAGACAGGGTGGCTGGAGA10120.11724144322826399No Hit
TTCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCATCCACCAGGGCCA10080.11677803831431828No Hit
CTCCTGCAGGGCCAGTCACAGTATTAGCACTTTCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT10010.1159670797149133No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG9850.11411346005913046No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT9800.11353420391669834No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA9410.10901600600572768No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC9400.10890015477724126No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9380.1086684523202684No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9340.1082050474063227No Hit
GTGCAGCCACAGTTCGTGTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC9300.10774164249237698No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCACAGTATTAGCA9050.1048453617802163No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9010.10438195686627062No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8990.10415025440929776No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8940.10357099826686562No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT8900.1031075933529199No Hit
CATACAACCCGTCCCTCAAGAGTCGCGTTAGCATATCATTAGACACGTCCAAGAACCAGTTCTCCCTGAACCTGA8870.10276003966746064No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCACAGTATTAGCACTTTCTTAGCCTGGTA8770.10160152738259637No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8660.10032716386924569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2400.044.333951
TAATGAT8450.044.04223670
TGGATTG10500.041.24336670
GTATCAA16000.038.18441
GGTATCA7800.037.8433721
TAACGGC400.002600737634.4870936
TTGTATA400.0026721234.2971954
TATTAGA601.3991214E-534.2912332
TATCAAC18300.033.54172
TCAACGC18950.032.2158364
ATCAACG19100.031.951723
CAACGCA19250.031.713775
AACGCAG19600.031.1474516
TGTTAGA4650.030.5583470
ATACCTA450.004739783230.5093821
ATTAGAG605.62279E-428.571063
AGAGTAC21800.028.3220711
CGCAGAG21650.028.0397388
CATTCCG500.00783652627.52550332
GTGGATT10750.027.51034269
Produced by FastQC (version 0.11.5)