FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780795
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT11640.14907882350681037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23050.040.8590851
TCGTATG2500.038.78180340
ACTCGAC3000.037.72765727
CTCGACG3050.036.00081628
TCGACGT3150.035.95878229
TATCAAC26900.035.772812
ATCAACG27150.035.1888923
TCAACGC27400.034.99284
CAACGCA27450.034.929065
GACTCGA3150.034.8244126
CGTATGC3000.034.68051541
AACGCAG28100.034.1210986
GGTATCA9450.034.066141
CGACGTC3300.033.29271330
CTCGTAT2650.032.6433639
ACGCAGA29800.032.176657
AGGGGGG5550.031.26007570
TATACAC4300.031.0575643
ACGAGAC3550.030.874822
TCTCGTA2800.030.87462838
Produced by FastQC (version 0.11.5)