FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167506
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40420.3462080708792931No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34520.29567299868266206No Hit
GGTCTATGCTGCATCCACTTTGCAGAGTGGGGTCCCGTCGAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA32320.27682941243985043No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30720.26312498608144197No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28680.2456518424744712No Hit
AGTCAGGACACAGCCTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC28000.23982746127214766No Hit
GCATAGACCAGGAGCTCAGGGGCTTTCCCTGGTTTCTGCCGATACCAGGC26210.22449563428367822No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25950.22226866500043682No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25800.22098387502933603No Hit
GCCTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG24820.21258991388481088No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23660.20265420477496476No Hit
GGACACAGCCTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23500.2012837621391239No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23340.1999133195032831No Hit
GACCCAGTCAGGACACAGCCTGGACATGAGGGTCCCCGCTCAGCTCCTGG22960.19665851824316105No Hit
CCATTAATGGCCTGCAGCCTGAAGATTTTGCAACTTACTTTTGTCAACAGGCTAACATTTTCCCCCCCGCGCTCA22590.1934893696477791No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22480.19254719033563852No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC22280.1908341370408375No Hit
GTCAGGACACAGCCTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC22080.18912108374603642No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21830.1869797671275351No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21790.1866371564685749No Hit
GGATATCAGCAGCTGGTTAGCCTGGTATCGGCAGAAACCAGGGAAAGCCCCTGAGCTCCTGGTCTATGCTGCATC21200.18158364924891177No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20050.1717335928038057No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACTGTGGTCCCTCCGCCGAAAGTGAGCGCGGGGGGGAAAATGTTAGC19920.17062010816218504No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACTGTGGTCC19700.16873574953790388No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19600.16787922289050336No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19560.16753661223154315No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18460.15811481911013733No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC18290.15665872380955645No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18270.15648741848007633No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18230.15614480782111612No Hit
CCACTGTGGTCCCTCCGCCGAAAGTGAGCGCGGGGGGGAAAATGTTAGCCTGTTGACAAAAGTAAGTTGCAAAAT17750.1520334799135936No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT17730.1518621745841135No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16850.14432474008698884No Hit
ATGTTAGCCTGTTGACAAAAGTAAGTTGCAAAATCTTCAGGCTGCAGGCCATTAATGGTGAGAGTGAAATCTGTC16740.14338256077484826No Hit
GATTTCACTCTCACCATTAATGGCCTGCAGCCTGAAGATTTTGCAACTTACTTTTGTCAACAGGCTAACATTTTC16550.14175516014478726No Hit
CTCTATCTCCTACAGATGCAGACACGGAAGATGGAGATTGGGTCATCTGG16480.1411555914916069No Hit
GTAGGAGATAGAGTCACCATCACTTGTCGGGCGAGTCAGGATATCAGCAGCTGGTTAGCCTGGTATCGGCAGAAA16480.1411555914916069No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16400.14047037017368647No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15840.1356738209482435No Hit
CCACAGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT15720.1346459889713629No Hit
GTCTCAGGTGGCTCCATCGGCAATGGTGGGTACTACTGGACCTGGATCCG15570.1333611990002621No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14860.12727985980371834No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14370.12308287923145578No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14260.12214069991931518No Hit
GAGATAGAGTCACCATCACTTGTCGGGCGAGTCAGGATATCAGCAGCTGG14250.12205504725457514No Hit
GTGGGGTCCCGTCGAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT14130.1210272152776945No Hit
GTAATAAACGGCCGTGTCCGCGGCAGTCACAGAGATCACCTTCAGGGAGA14100.12077025728347436No Hit
CTGCAAAGTGGATGCAGCATAGACCAGGAGCTCAGGGGCTTTCCCTGGTTTCTGCCGATACCAGGCTAACCAG13880.11888589865919319No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13840.11854328800023298No Hit
ATCTCCTACAGATGCAGACACGGAAGATGGAGATTGGGTCATCTGGATGTCGCATCTGGAACCTGGGAACCAGAG13720.11751545602335235No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13570.11623066605225155No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13380.11460326542219056No Hit
GACCAGGAGCTCAGGGGCTTTCCCTGGTTTCTGCCGATACCAGGCTAACC13300.11391804410427013No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13290.11383239143953007No Hit
GGACCCCACTCTGCAAAGTGGATGCAGCATAGACCAGGAGCTCAGGGGCTTTCCCTGGTTTCTGCCGATACCAGG13030.1116054221562887No Hit
CTCCTGGTCTATGCTGCATCCACTTTGCAGAGTGGGGTCCCGTCGAGGTT12950.11092020083836827No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12920.11066324284414812No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12800.1096354108672675No Hit
ATGCTGCATCCACTTTGCAGAGTGGGGTCCCGTCGAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA12710.10886453688460701No Hit
AGTCAGGATATCAGCAGCTGGTTAGCCTGGTATCGGCAGAAACCAGGGAAAGCCCCTGAGCTCCTGGTCTATG12470.10680887293084576No Hit
GGTTAGCCTGGTATCGGCAGAAACCAGGGAAAGCCCCTGAGCTCCTGGTC12450.10663756760136565No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12300.10535277763026485No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12270.10509581963604471No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12130.10389668232968396No Hit
GAGTGGGGTCCCGTCGAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATTAATGGCCTGCAGC12040.10312580834702348No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12010.10286885035280333No Hit
GCTGATATCCTGACTCGCCCGACAAGTGATGGTGACTCTATCTCCTACAG11970.10252623969384313No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11960.10244058702910305No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11830.10132710238748238No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)