FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393567
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16570.4210210713804765No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT5170.1313626396522066No Hit
CATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA4280.10874895506991185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8750.042.4142071
TATCAAC9550.038.4540672
TATACTT451.1502616E-437.4348955
TAGGCAA951.2789927E-436.75392570
TCAACGC10250.035.1660734
CAACGCA10300.034.9998135
AACGCAG10300.034.9998136
ATCAACG10400.034.9827843
ACGCAGA10750.033.5347027
TAATTCA552.3547569E-433.13881360
AGAGTAC11150.032.9318111
CGCAGAG10900.032.7599568
GACCGAT1103.0263572E-431.74202370
CAGAGTA11600.031.36387610
GCAGAGT11950.030.445279
GTGGTAT1251.6189006E-1029.6899471
AGTACGG11500.029.00390813
GAGTACG11950.027.9117112
TACGGGG6750.026.45399715
GTACGGG12850.025.43242514
Produced by FastQC (version 0.11.5)