FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340873
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATTACTGTCAGCACTATGATACCTCACCTATGTACACTTTTGGCCA14880.11097247837789262No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCTGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA13690.1020976632388004No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13470.10045694111224554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33150.037.52361
ATCAACG36450.034.4040833
CAACGCA37050.033.845675
TCAACGC36850.033.8454254
TATCAAC37350.033.4855772
AACGCAG38050.033.133076
AGAGTAC40800.030.982911
ACGCAGA41950.029.9719757
CGCAGAG42550.029.7086358
CTTATCG1655.3085314E-627.75446970
CAGAGTA46000.027.48048810
AGTACGG39750.026.60155713
GGTATCA19850.026.1247961
GCAGAGT49200.025.6931389
GAGTACG41250.025.55111512
CGCATAA1300.001180751125.1620270
ACCGATC2850.023.8118627
GTACGGG44950.023.29972514
CCGATCA2800.023.03381728
TACGGGA18200.022.72095715
Produced by FastQC (version 0.11.5)