FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275545
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10530.38215173565116406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9700.038.628811
GGTATCA4200.036.4967041
TATCAAC10900.034.0388532
TCAACGC10900.033.4082224
CAACGCA10850.033.2485125
AACGCAG11000.032.7951246
ATCAACG11050.032.646733
TAGTGTG800.003702597732.07081670
ACGCAGA11300.031.9244547
TACGGGT3000.031.78113715
AGAGTAC12000.030.91301311
CAGAGTA12300.030.15903710
CGCAGAG12250.029.7211068
AGTACGG10800.029.31123213
GCAGAGT12700.028.9411729
GAGTACG11050.028.64808312
TATAGGT605.8611354E-428.3605615
GTGGTAT1603.6379788E-1227.6766661
GTACGGG11500.027.23108314
TGGTATC1751.0913936E-1125.2860032
Produced by FastQC (version 0.11.5)