FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365960
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18960.13880347887200212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39550.042.025781
GGTATCA15600.038.8838461
TATCAAC44400.037.234332
ATCAACG44500.037.0547373
TCAACGC44600.036.742894
CAACGCA45650.035.900395
AACGCAG46250.035.5813036
ACGCAGA48350.033.8931277
CGCAGAG50600.032.2496228
AGAGTAC50650.032.15259611
CAGAGTA53100.030.73127410
GCAGAGT54400.029.9968879
AGTACGG44850.027.6906213
GAGTACG45150.027.58240512
GTATCGT500.00799146527.4164091
GTGGTAT5700.026.454431
GTACGGG49150.025.06013514
CGAGTTA700.00140055324.4377113
TACGGGG18600.023.17895515
TACGGGA15800.022.73884815
Produced by FastQC (version 0.11.5)