FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988971
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11430.1155746730692811No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24250.038.184411
ATCAACG27150.033.8171963
TCAACGC26950.033.683394
CAACGCA27250.033.5656785
TATCAAC28100.032.802462
AACGCAG28250.032.256256
GGTATCA11000.031.4892791
GTGGTAT5150.030.6326581
ACGCAGA29850.030.4125047
CGCAGAG31200.029.3146928
CAGAGTA31500.029.03550710
AGAGTAC31350.028.95882611
GCAGAGT32550.028.2041179
TGGTATC5800.026.585512
AGTACGG29500.026.13226313
GAGTACG29600.025.92822812
TTAACGG1501.0513759E-925.28039235
TACGGGG14550.025.20270515
GTACGGG33200.023.11673414
CGAACTA1658.2352926E-820.83034729
Produced by FastQC (version 0.11.5)