FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417559
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10700.041.4106861
TCAACGC12050.037.345894
ATCAACG12150.036.7513773
CAACGCA12550.035.584285
AACGCAG13000.034.3525166
TATCAAC13100.034.34842
GGTATCA4850.033.9942971
ACGCAGA14500.030.798817
CGCAGAG15450.028.9050318
TTTCGAT1106.222526E-428.06962870
AGAGTAC15850.027.95547911
CAGAGTA16650.026.82178510
GCAGAGT16750.026.6616549
TATACTG658.866871E-426.425015
TTATACC750.002041122422.9016784
AGTACGG17250.022.30424313
GAGTACG17350.022.17568812
GCTTAGT953.1535936E-421.6937271
CTTATAC800.002971331821.467753
CGTCAGG1004.316798E-420.7082334
Produced by FastQC (version 0.11.5)