FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417559
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10900.036.603161
TTAACGG400.002592171334.50613835
TCAACGC11850.033.8979644
GGTATCA4900.033.6924251
CAACGCA12200.032.9254845
AACGCAG12200.032.9254846
ATCAACG12250.032.783243
TATCAAC12650.031.4979082
GCGACTC553.3523608E-431.203943
CATTCGC450.00446769830.87655446
GTCCGAC450.004645287530.63156533
ACGCAGA13250.029.7980657
GTATACT605.5727677E-428.6104644
ATTCGCA500.00744090627.81586647
TTTCGAT1156.774394E-427.6641970
CGCAGAG14700.026.6252468
AGAGTAC15000.025.8638611
CAGAGTA15400.025.63794510
TAAGCGC851.4798583E-424.25798823
GCAGAGT16350.023.9382939
Produced by FastQC (version 0.11.5)