FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824005
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36480.44271576021990156No Hit
GAGTTACAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT26830.32560482035909977No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23760.28834776488006747No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22820.27694006711124325No Hit
GATCTATGGTGTATTAAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA21190.25715863374615444No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21030.2552168979557163No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20660.250726633940328No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19980.24247425683096582No Hit
GTATTAGCACCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCT19390.23531410610372508No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18240.22135788010995078No Hit
CCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT17820.2162608236600506No Hit
GTAGGAGACAGAGTCACCATCAGTTGCCGGGCAAGTCAGAGTATTAGCACCTATTTAAATTGGTATCAGCAGAAA17530.21274142753988143No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17020.2065521447078598No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16860.2046104089174216No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC16840.20436769194361687No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16690.20254731464008105No Hit
CTGTAACTCTGTTGACAGAAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGTTGATGGTGAGAGTGAAATCTGTC16300.19781433365088805No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16220.19684346575566894No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGGGGTA16220.19684346575566894No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15830.1921104847664759No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15680.19029010746294014No Hit
GTACTGTAACTCTGTTGACAGAAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGTTGATGGTGAGAGTGAAAT15600.18931923956772106No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15470.18774157923799006No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG15370.18652799436896622No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15320.18592120193445427No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15080.18300859824879703No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15010.18215908884048035No Hit
GTTACAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA14630.17754746633818969No Hit
GATTTCACTCTCACCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTACTTCTGTCAACAGAGTTACAGTACC14600.17718339087748253No Hit
CCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTACTTCTGTCAACAG14580.17694067390367776No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC14560.176697956929873No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCAGTTGCCGGGCAAGTCAGAGTATTAGCAC14430.17512029660014197No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14430.17512029660014197No Hit
CACCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTACTTCTGTCAACAGAGTTACAGTACCCCTCGGACGTT14230.1726931268620943No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13880.1684455798205108No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT13860.16820286284670602No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG13830.16783878738599886No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT13800.1674747119252917No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13650.16565433462175594No Hit
CTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT12880.15630973113027224No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12780.1550961462612484No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12580.1526689765232007No Hit
GTGCTAATACTCTGACTTGCCCGGCAACTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGA12090.14672241066498384No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11430.13871275052942642No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCAGTTGCCGGGCAAGTCAGA11340.13762052414730494No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11330.13749916566040254No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA11160.135436071383062No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11040.13397976954023338No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC11010.13361569407952623No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10700.12985358098555227No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10600.12863999611652843No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10410.1263341848653831No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10390.12609146789157832No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTTCTGTCAACAGAGTTACAGTACCCCTCGGACGTTCGGCCAAGGGA10010.12147984538928769No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC9800.1189313171643376No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9720.1179604492691185No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9610.11662550591319228No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9560.11601871347868035No Hit
GGGTACTGTAACTCTGTTGACAGAAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGTTGATGGTGAGAGTGAAAT9490.11516920407036366No Hit
ACAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA9450.1146837701227541No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9420.11431969466204694No Hit
GCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGGTGTATTAAATTTGCAAAGTGGGGTCCCATCAAGGTT9360.11359154374063264No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9120.11067894005497539No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9070.11007214762046348No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTA9030.10958671367285393No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9020.10946535518595155No Hit
CCTTGGTCCCTTGGCCGAACGTCCGAGGGGTACTGTAACTCTGTTGACAG9010.10934399669904915No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8930.10837312880383007No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8920.10825177031692768No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAACAGTCTGCAACCTGAAGATTTTG8910.1081304118300253No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8890.10788769485622052No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8780.1065527515002943No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCA8740.10606731755268474No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT8720.10582460057887999No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8710.1057032420919776No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8490.10303335538012512No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG8490.10303335538012512No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC8450.10254792143251559No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8450.10254792143251559No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8420.10218384597180843No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8310.10084890261588218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15800.044.6864171
TATCAAC17450.040.4045522
ATCAACG17500.040.07653
TCAACGC18400.038.2998434
CAACGCA18500.038.2777335
AACGCAG18650.037.969876
AGAGTAC19600.035.95495611
CGCAGAG19900.035.5848278
GTGGTAT1650.035.3126531
GCAGAGT20200.034.7176329
ACGCAGA20700.034.209577
AGTACGG19600.031.59532513
GAGTACG20250.030.58115412
TACGGGG8800.028.39045315
GTACGGG21900.028.12086914
TTGACTA500.007317155627.91480452
TACGGGA10800.027.8863115
ACGGGGA4000.027.38427416
ACGGGAT2050.026.71636616
TTGCTAC550.00919354326.63844967
Produced by FastQC (version 0.11.5)