FastQCFastQC Report
Wed 8 May 2024
EGAF00005761255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772460
Total Bases57.6 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCTGGACTGTCTGGCACAATAATAAATAGCCGTGTCTGCGGAGGTCAC24260.3140615695311084No Hit
GTCCAGCAGTGGCTATTACAGGGGAGGATTCTACTTTGAATACTGGGGCC22290.28855863086761774No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21180.2741889547678844No Hit
ACCTACTACACCCCGTCCCTCAAGAGTCGAGTCACCATTTCCATAGACAC20550.26603319265722497No Hit
GTCTCTGGTGACTCCATGAGAAACACTAATGACTACTGGGGCTGGATCCG20440.26460917070139556No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC20250.26214949641405383No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT19560.2532169950547601No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA18810.2435077544468322No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18560.24027134091085625No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17550.2271962302255133No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17450.22590166481112292No Hit
GTCTATTCTAGTGGCACTACCTACTACACCCCGTCCCTCAAGAGTCGAGT17270.2235714470652202No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC16940.2192993811977319No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16910.2189110115734148No Hit
CTATTAATACTCTCACTGGCCCGACAAGTGATGGTGACTCTGTCTCCCAC16110.20855448825829168No Hit
GTATTAATAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGAAAAGCCCCT16030.2075188359267794No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15960.20661264013670613No Hit
GTAGTAGGTAGTGCCACTAGAATAGACAGTCCCAATGCACTCCAGACCCT15680.202987856976413No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCGGGCCAGTGAGA15420.19962198689899802No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15410.19949253035755898No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15400.19936307381611995No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15340.19858633456748567No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15080.1952204644900707No Hit
AGAATAGACAGTCCCAATGCACTCCAGACCCTTCCCGGGGGACTGGCGGA15050.19483209486575356No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG14890.19276079020272896No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA14860.1923724205784118No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14750.1909483986225824No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14590.18887709395955776No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14250.18447557155063046No Hit
CACCTGCACTGTCTCTGGTGACTCCATGAGAAACACTAATGACTACTGGG14030.1816275276389716No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13950.1805918753074593No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13950.1805918753074593No Hit
CCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCG13230.1712710043238485No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCT13130.1699764389094581No Hit
GTCTGGCACAATAATAAATAGCCGTGTCTGCGGAGGTCACAGAGCTCAGT13030.1686818734950677No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12970.16790513424643347No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12530.16220904642311576No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA12510.1619501333402377No Hit
GTAATAGCCACTGCTGGACTGTCTGGCACAATAATAAATAGCCGTGTCTG12460.1613028506330425No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12430.16091448100872535No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12330.15961991559433497No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12260.1587137198042617No Hit
CTCCAGACCCTTCCCGGGGGACTGGCGGATCCAGCCCCAGTAGTCATTAG12130.1570307847655542No Hit
GTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTCTTCCTCCT11840.15327654506382207No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11690.1513346969422365No Hit
CCTTATGGATCAGGAGCTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAG11690.1513346969422365No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTG11610.15029904461072416No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11440.14809828340626052No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG11250.14563860911891877No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC11170.14460295678740648No Hit
GCATTGGGACTGTCTATTCTAGTGGCACTACCTACTACACCCCGTCCCTC10910.14123708670999144No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAATTGTGACATCC10850.14046034746135724No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10690.13838904279833258No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC10640.1377417600911374No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10500.13592936851099086No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10460.1354115423452347No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10460.1354115423452347No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA10460.1354115423452347No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10270.13295186805789297No Hit
GTCCCAATGCACTCCAGACCCTTCCCGGGGGACTGGCGGATCCAGCCCCA10250.1326929549750149No Hit
GGTCTGGAGTGCATTGGGACTGTCTATTCTAGTGGCACTACCTACTACAC10130.13113947647774643No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT10050.13010382414623411No Hit
GATGGGTCCTGTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTG10000.1294565414390389No Hit
GTGCCAGACAGTCCAGCAGTGGCTATTACAGGGGAGGATTCTACTTTGAA10000.1294565414390389No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG9910.12829143256608758No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT9890.12803251948320948No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9860.1276441498588924No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC9390.12155969241125753No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA9280.12013567045542813No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGG9250.119747300831111No Hit
ACTACACCCCGTCCCTCAAGAGTCGAGTCACCATTTCCATAGACACATCC9170.1187116484995987No Hit
GTGTAGTAGGTAGTGCCACTAGAATAGACAGTCCCAATGCACTCCAGACC9050.11715817000233023No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA9030.11689925691945213No Hit
GGCCAAAAGTGTACAAATAATTAAAATAGTGTTGGCAGTAATAAGTTGCA8970.1161225176708179No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC8920.1154752349636227No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8890.1150868653393056No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA8880.11495740879786656No Hit
GGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGG8870.11482795225642752No Hit
GTCCCTCACCTGCACTGTCTCTGGTGACTCCATGAGAAACACTAATGACT8830.11431012609067137No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC8800.11392175646635423No Hit
GAGTGAAGTCTGTCCCAGATCCACTGCCGCTGAACCTTGACGGGACACCA8630.1117209952618906No Hit
CCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTCCTGTCCC8590.11120316909613442No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGC8500.11003806022318308No Hit
GATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACAATTGGC8460.10952023405742693No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA8280.10719001631152422No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT8280.10719001631152422No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC8260.10693110322864613No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8250.10680164668720711No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGC8250.10680164668720711No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC8210.10628382052145094No Hit
GTCCTGTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCC8190.10602490743857287No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC8170.1057659943556948No Hit
GATCAGGAGCTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAGGCCAACC8160.10563653781425575No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT8140.10537762473137768No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC8120.1051187116484996No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA8120.1051187116484996No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG8090.1047303420241825No Hit
GTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGG8040.10408305931698728No Hit
GGACACCACTTGCTAAAGTAGACGCCTTATGGATCAGGAGCTTAGGGGCT8030.10395360277554826No Hit
CTACTACACCCCGTCCCTCAAGAGTCGAGTCACCATTTCCATAGACACAT7970.10317686352691402No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACAATTGGCACCTGGGAGC7900.10227066773684074No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7900.10227066773684074No Hit
GTGGGAGACAGAGTCACCATCACTTGTCGGGCCAGTGAGAGTATTAATAG7890.1021412111954017No Hit
GGCTATTACAGGGGAGGATTCTACTTTGAATACTGGGGCCCGGGAACCCT7860.10175284157108459No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC7780.10071718923957226No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG7740.10019936307381612No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)