FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772460
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCTGGACTGTCTGGCACAATAATAAATAGCCGTGTCTGCGGAGGTCAC22540.2917950444035937No Hit
ACCTACTACACCCCGTCCCTCAAGAGTCGAGTCACCATTTCCATAGACACATCCAATAACCACTTCTCCCTGCGA18920.24493177640266162No Hit
GTCTCTGGTGACTCCATGAGAAACACTAATGACTACTGGGGCTGGATCCG18120.23457525308753854No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18100.23431634000466045No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18080.23405742692178236No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17160.22214742510939076No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16900.21878155503197577No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16480.21334438029153613No Hit
GTCTATTCTAGTGGCACTACCTACTACACCCCGTCCCTCAAGAGTCGAGT16430.21269709758434094No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16000.20713046630246226No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA15930.206224270512389No Hit
GTCCAGCAGTGGCTATTACAGGGGAGGATTCTACTTTGAATACTGGGGCC15310.1981979649431686No Hit
CTATTAATACTCTCACTGGCCCGACAAGTGATGGTGACTCTGTCTCCCACAGATGCAGACAGGGTGGAAGGAGA15080.1952204644900707No Hit
GTATTAATAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGAAAAGCCCCTAAGCTCCTGATCCATAAGGCGTCTA14840.19211350749553374No Hit
GTAGTAGGTAGTGCCACTAGAATAGACAGTCCCAATGCACTCCAGACCCTTCCCGGGGGACTGGCGGATCCAGCC14400.18641741967221603No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCGGGCCAGTGAGA14390.186287963130777No Hit
AGAATAGACAGTCCCAATGCACTCCAGACCCTTCCCGGGGGACTGGCGGA14310.18525231079926469No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14220.18408720192631334No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14130.182922093053362No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14030.1816275276389716No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13570.1756725267327758No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13470.17437796131838543No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13470.17437796131838543No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13400.17347176552831214No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA13310.1723066566553608No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13220.17114154778240945No Hit
CACCTGCACTGTCTCTGGTGACTCCATGAGAAACACTAATGACTACTGGG13190.17075317815809232No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13150.17023535199233616No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13050.16894078657794578No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12960.16777567770499444No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12930.1673873080806773No Hit
GTCTGGCACAATAATAAATAGCCGTGTCTGCGGAGGTCACAGAGCTCAGT12220.15819589363850556No Hit
CCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCG12210.1580664370970665No Hit
GTAATAGCCACTGCTGGACTGTCTGGCACAATAATAAATAGCCGTGTCTG12030.15573621935116383No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAATTGTGACAT11940.15457111047821245No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11390.14745100069906533No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTT11180.1447324133288455No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11070.14330839137301607No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11070.14330839137301607No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11030.1427905652072599No Hit
CTCCAGACCCTTCCCGGGGGACTGGCGGATCCAGCCCCAGTAGTCATTAG11010.14253165212438185No Hit
CCTTATGGATCAGGAGCTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAGGCCAACCAACTATTAATACTCTCA10960.14188436941718663No Hit
GTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTC10740.1390363255055278No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10640.1377417600911374No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10630.1376123035496984No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10610.1373533904668203No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10430.13502317272091757No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10060.13023328068767315No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC9990.12932708489759986No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9840.1273852367760143No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9780.12660849752738004No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9630.12466664940579447No Hit
GGTCTGGAGTGCATTGGGACTGTCTATTCTAGTGGCACTACCTACTACAC9460.12246588820133081No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAATTGTGACATCCAGATGACCCAGTCTCCTTCCACC9430.1220775185770137No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9360.12117132278694043No Hit
GTCCCAATGCACTCCAGACCCTTCCCGGGGGACTGGCGGATCCAGCCCCA9350.12104186624550138No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9240.11961784428967195No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT9170.1187116484995987No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG9170.1187116484995987No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9040.11702871346089118No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9030.11689925691945213No Hit
GTGCCAGACAGTCCAGCAGTGGCTATTACAGGGGAGGATTCTACTTTGAATACTGGGGCCCGGGAACCCTGGTCA8950.11586360458793983No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG8880.11495740879786656No Hit
GGCCAAAAGTGTACAAATAATTAAAATAGTGTTGGCAGTAATAAGTTGCA8690.1124977345105248No Hit
GTGTAGTAGGTAGTGCCACTAGAATAGACAGTCCCAATGCACTCCAGACCCTTCCCGGGGGACTGGCGGATCCAG8540.11055588638893923No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8470.10964969059886595No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA8470.10964969059886595No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC8340.10796675556015846No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA8330.10783729901871941No Hit
GATGGGTCCTGTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCA8310.10757838593584133No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA8310.10757838593584133No Hit
ACTACACCCCGTCCCTCAAGAGTCGAGTCACCATTTCCATAGACACATCC8300.1074489293944023No Hit
GGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGACTCCATGAGAAACACTAATGA8240.10667219014576806No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG8230.10654273360432903No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8190.10602490743857287No Hit
GTCCCTCACCTGCACTGTCTCTGGTGACTCCATGAGAAACACTAATGACTACTGGGGCTGGATCCGCCAGTCCCC8000.10356523315123113No Hit
GAGTGAAGTCTGTCCCAGATCCACTGCCGCTGAACCTTGACGGGACACCA7980.10330632006835307No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA7970.10317686352691402No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7870.10188229811252364No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC7830.10136447194676747No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7820.10123501540532845No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA7810.1011055588638894No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)