FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772460
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCTGGACTGTCTGGCACAATAATAAATAGCCGTGTCTGCGGAGGTCAC22510.2914066747792766No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19740.25554721280066284No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19030.24635579835849106No Hit
ACCTACTACACCCCGTCCCTCAAGAGTCGAGTCACCATTTCCATAGACACATCCAATAACCACTTCTCCCTGCGA17810.23056210030292829No Hit
GTCTCTGGTGACTCCATGAGAAACACTAATGACTACTGGGGCTGGATCCG17060.22085285969500038No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16640.21541568495456073No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15530.20104600885482746No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15340.19858633456748567No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA14490.18758252854516738No Hit
GTAGTAGGTAGTGCCACTAGAATAGACAGTCCCAATGCACTCCAGACCCTTCCCGGGGGACTGGCGGATCCAGCC14460.18719415892085028No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14330.18551122388214275No Hit
GTCTATTCTAGTGGCACTACCTACTACACCCCGTCCCTCAAGAGTCGAGT14120.18279263651192296No Hit
AGAATAGACAGTCCCAATGCACTCCAGACCCTTCCCGGGGGACTGGCGGA14080.1822748103461668No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13980.18098024493177642No Hit
CTATTAATACTCTCACTGGCCCGACAAGTGATGGTGACTCTGTCTCCCACAGATGCAGACAGGGTGGAAGGAGA13820.1789089402687518No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13510.17489578748414156No Hit
CACCTGCACTGTCTCTGGTGACTCCATGAGAAACACTAATGACTACTGGG13380.17321285244543408No Hit
GTATTAATAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGAAAAGCCCCTAAGCTCCTGATCCATAAGGCGTCTA13090.16945861274370194No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCGGGCCAGTGAGA12740.16492763379333558No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12390.16039665484296922No Hit
GTCCAGCAGTGGCTATTACAGGGGAGGATTCTACTTTGAATACTGGGGCC12330.15961991559433497No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12260.1587137198042617No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12120.15690132822411518No Hit
GTCTGGCACAATAATAAATAGCCGTGTCTGCGGAGGTCACAGAGCTCAGT11970.15495948010252958No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11940.15457111047821245No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11910.15418274085389536No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11880.15379437122957823No Hit
GTAATAGCCACTGCTGGACTGTCTGGCACAATAATAAATAGCCGTGTCTG11580.14991067498640706No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11550.14952230536208996No Hit
GAGTGAAGTCTGTCCCAGATCCACTGCCGCTGAACCTTGACGGGACACCA11540.1493928488206509No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11510.14900447919633378No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA11280.1460269787432359No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11260.14576806566035783No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11200.14499132641172358No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11060.14317893483157704No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11040.14292002174869897No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGACGGGACACCACTTGCTAAAGT10870.1407192605442353No Hit
GTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTC10750.13916578204696683No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10710.13864795588121068No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10680.13825958625689358No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10600.13722393392538124No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTT10590.1370944773839422No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAATTGTGACAT10550.13657665121818605No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10490.13579991196955182No Hit
CCTTATGGATCAGGAGCTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAGGCCAACCAACTATTAATACTCTCA10340.13385806384796625No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC10170.13165730264350256No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10050.13010382414623411No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9930.12855034564896564No Hit
CCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCG9880.12790306294177045No Hit
CTCCAGACCCTTCCCGGGGGACTGGCGGATCCAGCCCCAGTAGTCATTAG9850.12751469331745333No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9530.12337208399140409No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG9360.12117132278694043No Hit
GGTCTGGAGTGCATTGGGACTGTCTATTCTAGTGGCACTACCTACTACAC9320.12065349662118427No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9210.11922947466535484No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9160.11858219195815965No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9040.11702871346089118No Hit
GTCCCAATGCACTCCAGACCCTTCCCGGGGGACTGGCGGATCCAGCCCCA9010.11664034383657407No Hit
GGCCAAAAGTGTACAAATAATTAAAATAGTGTTGGCAGTAATAAGTTGCA8990.11638143075369599No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8930.11560469150506175No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT8880.11495740879786656No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8830.11431012609067137No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG8730.11301556067628098No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA8650.11197990834476866No Hit
GATGGGTCCTGTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCA8520.11029697330606117No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8500.11003806022318308No Hit
GTGGGAGACAGAGTCACCATCACTTGTCGGGCCAGTGAGAGTATTAATAGTTGGTTGGCCTGGTATCAGCAGAAA8450.10939077751598789No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAATTGTGACATCCAGATGACCCAGTCTCCTTCCACC8310.10757838593584133No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA8270.10706055977008518No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT8230.10654273360432903No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8210.10628382052145094No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA8210.10628382052145094No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA8140.10537762473137768No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8080.10460088548274345No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8060.10434197239986537No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG8020.10382414623410921No Hit
GTGTAGTAGGTAGTGCCACTAGAATAGACAGTCCCAATGCACTCCAGACCCTTCCCGGGGGACTGGCGGATCCAG8020.10382414623410921No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACTGCCG7960.10304740698547497No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACAATTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG7960.10304740698547497No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7950.10291795044403594No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC7950.10291795044403594No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA7900.10227066773684074No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7890.1021412111954017No Hit
GTGCCAGACAGTCCAGCAGTGGCTATTACAGGGGAGGATTCTACTTTGAATACTGGGGCCCGGGAACCCTGGTCA7890.1021412111954017No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC7850.10162338502964555No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7840.1014939284882065No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG100.008821855134.7723870
TACCGAC150.002273044369.6208750
GCGATCG150.002389075768.75230433
ATACGAG150.00241069468.59630623
AATACGA150.002411933668.5874222
GATAGTG350.00476260457.75959470
GTGGTAT1250.054.884161
GACCCTA400.0080768250.53964270
GTATCAA12600.042.742131
TATCAAC14500.037.570732
TACGGGT952.3646862E-1036.0565715
ATCAACG15550.034.7999343
GTCAAAT400.002553933534.61456343
TCAACGC15650.034.360954
CAACGCA15800.033.817965
AACGCAG15750.033.7034846
AGTGTTT8800.031.39583870
AGAGTAC17250.031.16981111
CGCAGAG17100.031.042688
GCAGAGT17150.030.7524859
Produced by FastQC (version 0.11.5)