FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619503
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19430.31363851345352645No Hit
GTATAATGACTGGCAGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT19210.3100872796419065No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCA18140.29281536973993666No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14940.24116105975273727No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13390.21614100335268757No Hit
GTCTGGGACAGACTTCTCTCTCACCATCAGCAGCCTGCAGTCTGAAGATT11570.18676261454746787No Hit
CATCTATGGTGCATCGACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCT11310.18256570186100796No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGT10860.17530181451905802No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10570.17062064267646807No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC10390.1677150877396881No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10070.16254965674096816No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9760.15754564546095823No Hit
TCATTATACTGGTGACAGAAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGAGAAGTCTGTC9530.15383299193062824No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCGACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT9430.15221879474352829No Hit
GTCATTATACTGGTGACAGAAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGAGAAGT9420.15205737502481828No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9330.1506045975564283No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC9240.14915182008803832No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9180.14818330177577832No Hit
GTAATAAACGGCCGTGTCCGCGGCAGTCACAGAGTTCAACTTCAGGGAGA9080.14656910458867833No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9070.14640768486996833No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC9010.14543916655770836No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8830.1425336116209284No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8780.1417265130273784No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8700.14043515527769843No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8660.1397894764028584No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGGCACCCTGTCTGTGTCTCCAGG8630.13930521724672842No Hit
GTGTTAGCAGCGACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCGA8570.13833669893446843No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT8530.13769102005962844No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC8510.13736818062220843No Hit
GTCTCCAGGCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG8490.13704534118478845No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7950.12832867637444853No Hit
CCAGTGGCCCTGGTCGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCC7940.12816725665573855No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7320.11815923409571867No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCGACTTAGCCTGGTA7320.11815923409571867No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7290.11767497493958866No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7200.11622219747119869No Hit
GGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAC6640.10718269322343879No Hit
GAGTGTTAGCAGCGACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTC6550.10572991575504881No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC6500.10492281716149882No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6320.10201726222471884No Hit
ATGGTGCATCGACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCTCTCTCA6280.10137158334987885No Hit
CTGCTACTCTGGCTCCCAGATTCCACTGGAGAAATAGTGATGACGCAGTC6260.10104874391245885No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6200.10008022560019887No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAGT200.00756201651.3922544
GTATCAA14400.040.2161751
GGTATCA6150.037.3315661
GTGGTAT1850.037.045451
TACGGGT1400.036.7057915
TATCAAC16250.035.4182622
ATTAGAC400.002686278234.2587363
ATCAACG17100.033.858053
TCAACGC17150.033.562294
TGGTATC2050.033.423162
CAACGCA17650.032.6115155
AACGCAG17800.032.334096
CGCAGAG18650.030.8604188
AGAGTAC18550.030.65493811
TATACAC802.914041E-629.9763953
ACGCAGA19650.029.2899137
GCAGAGT19750.029.141619
CAGAGTA20800.027.50581410
GAGTACG18150.026.80081612
AGTACGG18200.026.5411113
Produced by FastQC (version 0.11.5)