FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619503
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGACTGGCAGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT19620.31670548810901644No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18490.2984650598947866No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCA16390.264566918965687No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13240.21371970757203756No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12510.20193606810620773No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11450.1848255779229479No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10470.16900644548936808No Hit
GTCTGGGACAGACTTCTCTCTCACCATCAGCAGCCTGCAGTCTGAAGATT10110.16319533561580815No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC10010.16158113842870817No Hit
CATCTATGGTGCATCGACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCT9980.16109687927257818No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9910.1599669412416082No Hit
TCATTATACTGGTGACAGAAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGAGAAGTCTGTC9850.1589984229293482No Hit
GTCATTATACTGGTGACAGAAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGAGAAGT9500.15334873277449826No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9480.15302589333707825No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC9400.1517345355873983No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGT9390.1515731158686883No Hit
GTAATAAACGGCCGTGTCCGCGGCAGTCACAGAGTTCAACTTCAGGGAGA9140.14753762290093833No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9090.14673052430738834No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC9050.14608484543254835No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8920.14398638908931838No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8750.14124225387124842No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT8750.14124225387124842No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8300.13397836652929848No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCGACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT8090.13058855243638853No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGGCACCCTGTCTGTGTCTCCAGG7990.12897435524928855No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC7990.12897435524928855No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7910.12768299749960854No Hit
GTGTTAGCAGCGACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCGA7820.1262302200312186No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7600.12267898621959861No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7570.12219472706346862No Hit
CCAGTGGCCCTGGTCGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCC7530.12154904818862863No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7450.12025769043894864No Hit
GTCTCCAGGCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG7290.11767497493958866No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7170.1157379383150687No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7050.11380090169054871No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCGACTTAGCCTGGTA6860.11073392703505876No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6840.11041108759763875No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6830.11024966787892874No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT6770.10928114956666876No Hit
GACTTCTCTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTTCTGTCACCAGTATAATGACTGG6230.10056448475632887No Hit
GGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAC6230.10056448475632887No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC150.002439655468.3884053
CGTGTAT400.00736058651.7381570
GTGGTAT1900.048.6742741
TGGTATC2100.045.625422
GGTATCA5550.042.5838971
GTATCAA14350.039.6228261
CAAGCGT350.00140760939.079094
TATCAAC15800.036.168252
ATCAACG15800.036.1419753
TCAACGC16050.035.5790144
CAACGCA16250.034.7258765
AACGCAG16450.034.719486
GCGGTAC400.00253359534.6699444
GGTGTTA400.002657989334.33276433
ACGGGTA501.9566265E-434.2052516
TACTGAT3500.033.5066170
CGCAGAG17900.032.2891278
AGAGTAC17900.032.09806411
GCAGAGT18450.031.3265789
ACGCAGA19000.030.2397587
Produced by FastQC (version 0.11.5)