FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953729
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10250.10747287751552066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24400.039.5912971
GGTATCA9950.037.5270041
TCAACGC28250.033.675274
ATCAACG28350.033.444553
CAACGCA28500.033.2615475
TATCAAC29200.032.945072
AACGCAG29350.032.5314646
ACGCAGA31450.030.3592557
CGCAGAG32000.029.8358928
AGAGTAC32200.029.44419711
CAGAGTA32850.028.9612310
GCAGAGT34350.027.7947149
GCGCATT500.00803932227.38204411
AGTACGG29150.026.65404713
GAGTACG28950.026.60172712
TACGGGG13350.023.84391415
GTGGTAT4350.023.6250321
GTACGGG33100.023.36987314
TACGGGA9200.021.20620215
GTATAAC1004.5705747E-420.537613
Produced by FastQC (version 0.11.5)