FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004300
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37580.37419097879119784No Hit
GTGTTGATGGTGAGAGTGAACTCTGTCCCAGATCCACTGCCACTGAACCT30760.30628298317235886No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27610.27491785323110624No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26710.2659563875336055No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25730.25619834710743805No Hit
CCATCAACACTCTGCAACCTGGAGATTTTGCAACTTACTACTGTCAACAG25560.2545056258090212No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTCTTCATTTGCAGAAACA24230.24126257094493675No Hit
GAGTTCACTCTCACCATCAACACTCTGCAACCTGGAGATTTTGCAACTTACTACTGTCAACAGAGTTTCAGTGGC23980.23877327491785322No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22610.22513193268943543No Hit
GTAGGAGACAGAGTCACAATCACTTGCCGGGCAAGTCAGGGCATTAGCAACTATTTAAATTGGTATCAGCAGAAA21410.21318331175943445No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACAATCACTTGCCGGGCAAGTCAGGGCATTAGCAA20640.20551627999601713No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19670.195857811410933No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19200.19117793488001592No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCCTGGCCGAACGTC18740.18659763019018222No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC18560.18480533705068206No Hit
GTGTATTACTGTGCGAAGGCGGGGGTCGTTCGAGGTTCGGGGAGTTATTGGTCAAGGAACGCTGGTGCGAATTA18370.18291347207009856No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18360.18281390022901522No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18250.1817186099770985No Hit
GATCTATGCAGCATCCACTTTGCAAAGTGAGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCA18130.18052374788409836No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18110.1803246042019317No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCCTGGCCGAACGTCCAAAGGCCA17890.17813402369809817No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT17540.1746490092601812No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16870.16797769590759734No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16770.1669819774967639No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCTCA16680.16608583092701384No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16410.16339739121776362No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16320.16250124464801352No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16150.16080852334959672No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14670.14607189086926217No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14570.14507617245842877No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14310.14248730459026188No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT14170.1410932988150951No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC14120.14059543960967838No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13850.13790699990042815No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCTCACTTTGCAAAGT13720.13661256596634472No Hit
ACTTACTACTGTCAACAGAGTTTCAGTGGCCTTTGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAACGAA13540.13482027282684458No Hit
GTAGTAAGTTGCAAAATCTCCAGGTTGCAGAGTGTTGATGGTGAGAGTGAACTCTGTCCCAGATCCACTGCCA13440.13382455441601115No Hit
CTCCTGATCTATGCAGCATCCACTTTGCAAAGTGAGGTCCCATCAAGGTT13440.13382455441601115No Hit
GTCTGCATCTGTAGGAGACAGAGTCACAATCACTTGCCGGGCAAGTCAGG13380.13322712336951112No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13360.13302797968734442No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13150.13093697102459426No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12670.12615752265259383No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12630.12575923528826047No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT12590.1253609479239271No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12470.12416608583092702No Hit
CTCCAGCCCCGTCCCTGGAGCCTGGCGGACCCAGCCCATCCAATAGTTTC12290.12237379269142686No Hit
GTCCAAAGGCCACTGAAACTCTGTTGACAGTAGTAAGTTGCAAAATCTCC11990.11938663745892661No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11890.1183909190480932No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11880.11829134720700986No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11700.1164990540675097No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT11600.11550333565667628No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11520.11470676092800956No Hit
GTGAGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCACT11500.11450761724584287No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11380.11331275515284278No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11370.11321318331175945No Hit
ATGCAGCATCCACTTTGCAAAGTGAGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCACTCTCA11270.11221746490092603No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACAATCA11200.11152046201334262No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10980.10932988150950912No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10970.10923030966842576No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10940.10893159414517575No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10790.10743801652892562No Hit
GCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCAGCATCCACTTTGCAAAGTGAGGTCCCATCAAGGTT10680.10634272627700887No Hit
ATCTGGGACAGAGTTCACTCTCACCATCAACACTCTGCAACCTGGAGATT10660.10614358259484218No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC10280.10235985263367518No Hit
GGGCTGGAGTGGGTGGCCAACATAAAACAAGATGGAAGTGAGAAACACTA10240.10196156526934184No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG200.00524282856.3806368
CTCGTAT1600.041.05888739
CTTATAC1700.040.458751
CGCTAAT1550.040.0443932
TATTGGC350.001256308539.99594557
GTATCAA17900.039.9614941
GTGGTAT2650.038.9320071
TCGTATG1600.038.9232340
TCGCTAA1650.037.5968131
GCTAATC1650.035.53647633
TATCAAC20450.035.3095782
AATCTCG1800.034.52059636
CTCGCTA1800.034.44311530
ATCTCGT1850.033.6077737
ATCAACG21300.033.577653
TCTGGAG10550.033.28116670
TCAACGC21550.033.1864624
CAACGCA21750.032.88135
AACGCAG22200.032.213186
TATACAC2150.031.9858653
Produced by FastQC (version 0.11.5)