FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501473
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14150.039.3383371
GTATCAA31550.038.881
TCGTATG952.1100277E-1036.44558740
ATCAACG36300.033.4104353
TATCAAC36600.033.0438082
CAACGCA37100.032.6889045
TCAACGC37250.032.557274
AACGCAG39950.030.3518496
GTGGTAT5250.029.451721
ACGCAGA41400.029.3717737
CGCAGAG43000.028.2779278
AGAGTAC43500.027.95289611
CAGAGTA44200.027.51020210
GCAGAGT46500.026.1494839
AGTACGG38100.025.33343913
GAGTACG39700.024.48548512
TGGTATC6900.023.403312
CTCGTAT1352.5878944E-723.06594839
CGTTAAC1102.8759701E-522.32072457
AATAGCG2700.022.23194962
Produced by FastQC (version 0.11.5)