FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1074559
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27600.039.001611
GGTATCA11300.038.104231
CTCGTAT2600.033.21401239
TATCAAC32800.033.0237272
ATCAACG32800.032.9195483
TCAACGC32950.032.769694
CAACGCA33000.032.720045
AACGCAG33950.032.1049046
TCGTATG2750.031.44671440
CGTATGC2850.030.38053141
ACGCAGA37400.029.0519967
AGAGTAC37450.028.83073611
CGCAGAG37950.028.4508828
GTGGTAT4400.027.9595991
CAGAGTA39150.027.666110
TCTCGTA3200.026.95341938
AGTACGG31200.026.72242713
GAGTACG31450.026.50877612
GCAGAGT40800.026.4635059
CTTATAC3500.026.3619041
Produced by FastQC (version 0.11.5)