FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688317
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18150.26368664438042355No Hit
CACTATCACCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTTCCCGCTCACTTT14610.2122568525839112No Hit
GGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCATC13830.20092486456095085No Hit
GGCTAACAGTTTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT12640.1836363187310498No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGGTGATAGTGAGAGTGAAATCTGTC12390.18000427128779325No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12270.17826088851503014No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11280.16387798063973433No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10920.15864783232144491No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10540.153127120207695No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10240.14876866327578717No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9900.1438290787529583No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG9870.14339323305976753No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGGAAA9080.1319159631390769No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAA8840.12842919759355065No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8610.12508771394575466No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8500.12348961307072177No Hit
GATCTATGCTGCATCCACTTTGCAAGATGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA8450.12276320358207046No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAAGATGGGGTCCCATCAAGGTT8410.12218207599114941No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8390.12189151219568892No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8240.11971228372973498No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8200.11913115613881395No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT8040.11680664577512978No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7980.1159349543887482No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG7810.11346516212733376No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT7760.11273875263868247No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7700.1118670612523009No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7450.10823501380904438No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7200.10460296636578786No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7090.10300486549075498No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7060.1025690197975642No Hit
GGTGATAGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTG6930.1006803551270708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2200.045.1991161
GTATCAA16100.039.6133581
TATCAAC18200.034.8212472
ATCAACG18500.034.246623
CAACGCA18450.034.1438875
TCAACGC18650.033.780184
AACGCAG19050.033.0684856
CGCAGAG20000.031.4977348
AGAGTAC20050.031.250711
CAGAGTA20350.030.78776610
ACGCAGA20650.030.6720777
CGGTTGG952.9000512E-729.38162452
GCAGAGT21500.029.1409789
ACTAGCC605.658841E-428.538853
TCGGAGC1153.8244252E-828.09839466
AGCGCTG2150.027.0708545
GAGTACG19900.026.84464612
AGTACGG19850.026.7397513
ATTTCGG1057.185299E-726.48532348
GTGCTAC658.9691376E-426.376141
Produced by FastQC (version 0.11.5)