FastQCFastQC Report
Wed 8 May 2024
EGAF00005761277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600884
Total Bases44.9 Mbp
Sequences flagged as poor quality0
Sequence length21-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22760.37877527110057846No Hit
GTATAGGGGCCTGGCTTGGCCTCTGCTGGTACCAATAAGCATATTGCTTT19740.32851598644663527No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18360.30554982326039637No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC18170.30238781528547937No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC17320.2882419901345351No Hit
CTATTACTGTCAGTCAGCAGACAGCAGTGGCACTTTTCGGAGAGTGTTCG17250.28707703982798677No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17040.28358218890834175No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCTCCGAAAAGTG16670.2774245944308718No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA16250.2704348925915817No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA15590.2594510754155544No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCT15370.2557898030235453No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA15080.25096358032498783No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT15050.2504643159078957No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC14150.23548638339513117No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC13430.22350403738491958No Hit
GGCTTGGCCTCTGCTGGTACCAATAAGCATATTGCTTTGGCAATGCATCT12940.2153493852390811No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12780.2126866416812563No Hit
TCAGTGGAGTCCGGGCAGAGGACGAGGGTGACTATTACTGTCAGTCAGCA12730.21185453431943604No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT12520.208359683399791No Hit
GTGTCAGTGTCCCCGGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC12420.20669546867615046No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCG12420.20669546867615046No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12260.20403272511832568No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC12250.20386630364596162No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT12090.20120356008813683No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA11880.19770870916849176No Hit
GTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGC11560.19238322205284214No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT11520.19171753616338594No Hit
GGACGAGGGTGACTATTACTGTCAGTCAGCAGACAGCAGTGGCACTTTTC11510.1915511146910219No Hit
CAGTAATAGTCACCCTCGTCCTCTGCCCGGACTCCACTGATGGTCAACGT11150.18555994168591608No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10980.18273077665572723No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG10960.18239793371099913No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10720.17840381837426192No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTC10630.17690602512298548No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10500.1747425459822528No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10490.17457612450988874No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10110.16825210856005485No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG10020.1667543153087784No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGG10010.16658789383641434No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC9940.165422943529866No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA9780.1627601999720412No Hit
GGCCAAGCCAGGCCCCTATACTATTAATATATGAGGACACTGAGAGGCCC9740.162094514082585No Hit
CTGCTGGTACCAATAAGCATATTGCTTTGGCAATGCATCTCCAGAGCAGG9740.162094514082585No Hit
CCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTCCCCGGGACAGACG9680.16109598524840069No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT9650.16059672083130855No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT9400.1564361840222073No Hit
CACTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAG9280.1544391263538387No Hit
CAGTCACGTTGACCATCAGTGGAGTCCGGGCAGAGGACGAGGGTGACTAT9190.15294133310256222No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGA9000.14977932512764527No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACA8920.14844795334873287No Hit
GACTATTACTGTCAGTCAGCAGACAGCAGTGGCACTTTTCGGAGAGTGTT8910.1482815318763688No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8780.14611805273563616No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC8440.14045972267525844No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTAT8420.14012687973053037No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC8360.13912835089634604No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8190.1362991858661572No Hit
GAGGACGAGGGTGACTATTACTGTCAGTCAGCAGACAGCAGTGGCACTTT8170.1359663429214291No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCG8150.135633499976701No Hit
CTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGG8140.13546707850433692No Hit
GCAGACAGCAGTGGCACTTTTCGGAGAGTGTTCGGCGGAGGGACCAAGCT8100.13480139261488075No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8070.13430212819778858No Hit
CCCCGGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAG8020.13347002083596834No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCTCCGAAAAGTGC7940.13213864905705594No Hit
GGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTG7870.1309736987505076No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAAGTCC7780.12947590549923113No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7780.12947590549923113No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTG7770.1293094840268671No Hit
GTAATAGTCACCCTCGTCCTCTGCCCGGACTCCACTGATGGTCAACGTGA7760.12914306255450303No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTC7750.128976641082139No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC7720.12847737666504683No Hit
GGGACAACAGTCACGTTGACCATCAGTGGAGTCCGGGCAGAGGACGAGGG7680.12781169077559065No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAG7640.12714600488613442No Hit
GTGGAGTCCGGGCAGAGGACGAGGGTGACTATTACTGTCAGTCAGCAGAC7560.12581463310722205No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCC7490.12464968280067366No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7460.12415041838358153No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA7410.12331831102176127No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTC7390.12298546807703317No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC7310.12165409629812077No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7250.12065556746393648No Hit
CCCTCGTCCTCTGCCCGGACTCCACTGATGGTCAACGTGACTGTTGTCCC7190.11965703862975216No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA7180.11949061715738811No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTG7070.11765998096138355No Hit
CAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAGTCACG7030.11699429507192736No Hit
CTCCACTGATGGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAAT6950.11566292329301495No Hit
GCTTGGTCCCTCCGCCGAACACTCTCCGAAAAGTGCCACTGCTGTCTGCT6900.11483081593119471No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA6880.1144979729864666No Hit
GCATATTGCTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGCCGTCTG6880.1144979729864666No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCG6840.1138322870970104No Hit
CCCCTATACTATTAATATATGAGGACACTGAGAGGCCCTCAGGGATCCCT6790.11300017973519015No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGG6690.11133596501154966No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT6690.11133596501154966No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAA6640.11050385764972939No Hit
GCTCAGGGACAACAGTCACGTTGACCATCAGTGGAGTCCGGGCAGAGGAC6540.10883964292608889No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA6500.10817395703663271No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGT6470.10767469261954055No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAAAGCAATATGCTTATTGGTAC6460.1075082711471765No Hit
GAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGAC6320.10517837053407979No Hit
CACTGACACCGAGGGCGGCTGTGTCAGCTCATAGGAGGCCTCAGAGCCTG6260.1041798416998955No Hit
GCTCTGGAGATGCATTGCCAAAGCAATATGCTTATTGGTACCAGCAGAGG6260.1041798416998955No Hit
GGCCTGGCTTGGCCTCTGCTGGTACCAATAAGCATATTGCTTTGGCAATG6200.10318131286571118No Hit
ACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCT6160.10251562697625499No Hit
GCCGAACACTCTCCGAAAAGTGCCACTGCTGTCTGCTGACTGACAGTAAT6150.10234920550389094No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT6130.10201636255916284No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC6110.10168351961443473No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGC6090.10135067666970662No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT6090.10135067666970662No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG6080.10118425519734257No Hit
GGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACT6070.10101783372497854No Hit
ACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTC6040.10051856930788639No Hit
CTATTAATATATGAGGACACTGAGAGGCCCTCAGGGATCCCTGAGCGATT6010.10001930489079423No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)