FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862372
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31370.36376412963315136No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27300.3165687197636287No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23550.273084005510383No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23390.27122865770224447No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22330.2589369784733271No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22300.2585891007593011No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC22210.2575454676172232No Hit
GTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC21980.2548784051430241No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20630.23922390801185567No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT20090.2329621091593883No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19690.2283237396390421No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19540.22658435106891225No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19500.22612051411687764No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18970.21997467450241892No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18940.21962679678839292No Hit
CTATAAAGCTGTTGACAGGAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC18860.2186991228843237No Hit
GCTTTATAGTTTTCCTCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCAT18490.21440863107800345No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAA18470.21417671260198615No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT18410.21348095717393423No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAT17470.20258078880112063No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17460.202464829563112No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17450.20234887032510332No Hit
GATCTATGCTGCATCCACTTTACAAAATGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA17070.1979424192807744No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17060.19782646004276577No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16850.195391316044584No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16790.1946955606165321No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16620.19272425357038495No Hit
GCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC16270.188665680240082No Hit
CTCCTGATCTATGCTGCATCCACTTTACAAAATGGGGTCCCATCAAGGTT16190.18773800633601276No Hit
GTAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA15680.18182408519757134No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTCCTGTCAACAGCTTTATAGTTTT15680.18182408519757134No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15030.17428673472700876No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14800.1716196722528097No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC14770.17127179453878374No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14690.1703441206347145No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCC14260.1653578734003423No Hit
GTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAATAACTGCTA14100.16350252559220382No Hit
ATGCTGCATCCACTTTACAAAATGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA14100.16350252559220382No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14040.1628067701641519No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT13940.16164717778406534No Hit
ACCTTGATGGGACCCCATTTTGTAAAGTGGATGCAGCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTG13730.15921203378588358No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13550.1571247675017278No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13290.15410982731350276No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12610.1462245991289142No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12600.14610863989090556No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12210.141586229608568No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12020.13938300408640356No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11980.13891916713436894No Hit
CAGTAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA11980.13891916713436894No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTG11870.13764361551627372No Hit
GGCCAAAAGTCCGAGGAAAACTATAAAGCTGTTGACAGGAATAAGTTGCA11750.13625210466016988No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11650.13509251228008332No Hit
ATATTACTGTGCGAGGGGGAAATATTGTGGTGGGATCACCTGCAATGAGT11610.13462867532804867No Hit
GGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCATC11580.1342807976140227No Hit
ACTTATTCCTGTCAACAGCTTTATAGTTTTCCTCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAA11550.13393291989999676No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11320.1312658574257977No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11170.12952646885566788No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11090.12859879495159862No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10850.1258157732393909No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10820.12546789552536491No Hit
GGAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG10800.1252359770493476No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10750.12465618085930434No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10710.1241923439072697No Hit
GTCTCCTACAGATGCAGACAGGAAGGATGGAGACTGGGTCAACTGGATGTCACATCTGGCACCTGGGAGCCAGAG10570.12256891457514854No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10510.12187315914709662No Hit
ATGCAGCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAATAACTGCTAATGCC10410.12071356676701006No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10350.12001781133895813No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC10230.11862630048285426No Hit
GCCACAGTTCGCTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTCCGAGG10080.11688691191272445No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCA10030.11630711572268115No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9880.11456772715255134No Hit
GTATCAACGCAGAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT9830.11398793096250806No Hit
CCCCTAAACTCCTGATCTATGCTGCATCCACTTTACAAAATGGGGTCCCA9830.11398793096250806No Hit
CTATATTACTGTGCGAGGGGGAAATATTGTGGTGGGATCACCTGCAATGA9800.11364005324848209No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9720.11271237934441286No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9670.11213258315436957No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG9590.11120490925030033No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9590.11120490925030033No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9530.11050915382224841No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9500.11016127610822245No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9360.10853784677610126No Hit
GCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGA9340.10830592830008395No Hit
CTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTCCTGTCA9180.10645058049194547No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT9170.10633462125393682No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9010.10447927344579833No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8970.10401543649376371No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8970.10401543649376371No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8960.10389947725575506No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTC8950.1037835180177464No Hit
GTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTG8800.10204412944761657No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8770.1016962517335906No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAACG150.002052604271.4353964
GTGGTAT1800.055.1752741
TGTGCGT200.007588096451.34923614
TCGCTAT306.5401633E-445.66759524
TCGTATG803.45608E-1143.11853840
GTGTATA803.8198777E-1142.7910313
GCCTATC856.91216E-1140.39100633
CTTATAC955.456968E-1239.65411
GTATCAA13000.038.4617041
CGTATGC901.2187229E-1038.3544841
TGTATAA901.3278623E-1038.03647214
GTATGCC1009.094947E-1238.0064942
GTAGTTT700.001901681736.75554370
ACGAGAC952.382876E-1036.04709222
TATCAAC14500.034.953252
ATGTGTA1004.1472958E-1034.23282211
GTATAAG1004.1472958E-1034.23282215
ATCAACG15550.032.8132823
TATCTCG1056.657501E-1032.75271236
TATAAGA1056.9849193E-1032.6045816
Produced by FastQC (version 0.11.5)