FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45665
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT10032.196430526661557No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT1750.3832256651702617Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT1310.28687178364173876Illumina PCR Primer Index 10 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.18175845833789556No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.16204970984342495No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.14891054418044453No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.1379612394612942No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.13577137851746415No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.12482207379831381No Hit
CCATTTCTCTGCGCAGGCACCCGTACTCTGCGTTGATAC550.12044235191065367No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.12044235191065367No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.11606263002299354No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT500.10949304719150334No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT500.10949304719150334No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.10730318624767327No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.10292346436001315No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)