FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45665
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18434.035913719478813No Hit
GTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGG540.11825249096682361No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCAAT100.009586308130.8057470
AAACGGG150.002153585270.4577938
GGTCGTG300.002875773565.4028770
CTCTCTC200.006058992454.26282546
AGCCAGC200.00708556152.16109535
GTATCAA6850.034.582511
GTCCTCA502.4946628E-432.7375721
AACGCAG6950.032.684176
GGGTCCT400.00337786132.62940619
ACCCGTA400.00337786132.62940619
CACCCGT400.00337786132.62940618
ACGGGAA701.4761517E-632.5935116
AAAATTA500.003665893632.07307468
CAGAGTA7250.031.77931610
ACGCAGA7200.031.5493037
AGAGTAC7200.031.54930311
TATCAAC7650.031.390232
GCAGAGT7250.031.331729
CAACGCA7650.030.1176535
CTCAGTC554.250466E-429.8935524
Produced by FastQC (version 0.11.5)