FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815662
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16540.20278007311852214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20200.039.4246861
GGTATCA8050.037.363751
TATCAAC23650.033.6549222
ATCAACG23950.032.92793
TCAACGC24000.032.8613134
CAACGCA24550.032.264185
AACGCAG25250.031.2345126
AGAGTAC26300.029.73516811
ACGCAGA26650.029.4655657
GTGGTAT2950.028.9655441
CGCAGAG27100.028.85038
CAGAGTA27750.028.29756210
GCAGAGT28200.027.7249349
AGTACGG23850.026.05995613
GAGTACG24400.025.47253812
GTATTAG3000.023.9255391
GTATCTA750.002103685422.7862281
GTACGGG27200.022.72481314
CGCGGTA1704.1673047E-922.44389343
TGGTATC3700.022.1581442
Produced by FastQC (version 0.11.5)