FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314101
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22610.17205679015539901No Hit
CATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA21860.16634946628912084No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT17260.13134454657594813No Hit
GCATAGATGAGGCGCCTAGGGGCTTCCCCTGGTTTCTGCTGATACCAGAC16490.1254850274065692No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16410.12487624619416621No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15610.11878843407013616No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14960.11384208671936175No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC14950.11376598906781138No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT13930.10600402860967306No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13910.10585183330657233No Hit
CTGTAAGTCTGCTGACAATAGTAAGTCGCGAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC13260.10090548595579792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31150.039.2204131
TCGTATG1750.035.5489240
TATCAAC34850.035.0550732
GTGGTAT3950.032.830211
ATCAACG37450.032.7124633
CGTATGC1800.032.6816741
TCAACGC37800.032.408344
CAACGCA38350.031.8545725
CGCTAAT1850.031.4885632
AACGCAG39350.031.0450536
TCGCTAA1900.030.64235531
ACGAGAC2200.029.48716222
AGAGTAC42950.028.36345911
CGCAGAG43550.028.1293968
CAGAGTA44350.027.69893310
ATTCGCG500.00804319427.38009530
ACACTCG2550.026.80027627
GCAGAGT45750.026.7767269
ACGCAGA46200.026.5159157
AGTACGG36900.025.9856813
Produced by FastQC (version 0.11.5)