FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066256
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23280.21833405861256583No Hit
GTATAACAGTGCCCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT20830.19535646223796163No Hit
GGAGTACACAGCCGTGTCCGCGGCGGTCACAGAGGTCAAGTTCAGGGAGA20360.19094851517834366No Hit
CTGTTATACTTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGAGAAATCTGTC19550.18335184045857655No Hit
GTCTACAGTGGGTCCTTCAGAGGTTACTACTGGAGCTGGATCCGCCAGCC17020.15962395522276077No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16390.15371543044071967No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15480.14518089464443812No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15330.14377410302966642No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14480.13580228387929352No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14340.13448927837217328No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14260.13373898951096172No Hit
AGTCAGGACATTAGCCATTATTTGGCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTATT13780.12923725634369232No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13710.12858075359013219No Hit
GTGTACTCCTGTGCGAGAGGCCGCGTCCGATGGCCCCCCTTTGACTACTGGGGCCAGGGAAGCCTGGTCACCGT13630.12783046472892062No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13190.12370387599225702No Hit
GATCTATTCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCT12390.11620098738014134No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12240.11479419576536967No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT12120.11366876247355233No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11950.11207439864347774No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11850.11113653756696328No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG11850.11113653756696328No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11710.10982353205984303No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTT11420.10710373493795111No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11180.10485286835431641No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG11180.10485286835431641No Hit
GTTATACTTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGC11160.10466529613901351No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11090.1040087933854534No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10900.10222685734007594No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10860.10185171290947015No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)