FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654450
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCGCGGCGGTCACAGAGGTCATCCTCAGGGTAA16440.25120330048132017No Hit
GTCTATTACTGCGCGACCGACACACTAGGGTTTTGTAGAGGTGCCATCTG12730.19451447780579112No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10420.15921766368706547No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10350.1581480632592253No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9370.1431736572694629No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9310.14225685690274276No Hit
GTCTCTGGTGGGTCCTTCACTGCTTACTACTGGCGTTGGATCCGTCAGCC9110.13920085568034227No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8960.13690885476354192No Hit
GTAGGGGACAGCGTCACCATCACTTGCCGGGCGAGTCAGGGCATTAGCAATTCGTTAGCCTGGTATCAGCAGAAA8650.13217205286882114No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8110.12392084956833983No Hit
GATCTATGGTGCGTACATTTTGCAATCAGGGGTCCCATCTCGGTTCCGTGGCAGTGGATCTGGGACAGATTTCA8090.12361524944609979No Hit
CCATAGATCAGGAGGTCAGGAACTTTCCCTGGTTTCTGCTGATACCAGGC7620.11643364657345863No Hit
GTTATAGTTCTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGC7610.1162808465123386No Hit
CTGTTATAGTTCTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC7520.11490564596225838No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC7150.1092520437008175No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7070.10802964321185728No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7020.10726564290625716No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCGGCTCCCAAATGGGTC6870.1049736419894568No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6820.10420964168385667No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG6800.10390404156161662No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG6780.10359844143937658No Hit
CTATAACAGTGCCCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAACTGTGGCTGCACCAT6770.10344564137825656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12300.038.3030931
TCGTATG1200.037.37893340
TATACAC1500.036.410133
GTGGTAT2350.034.866091
TATCAAC14250.033.298652
TTATACA1700.032.129042
CGTATGC1400.032.08369441
TCAACGC15200.030.7612554
ATCAACG15250.030.6650893
TACTGAT6550.030.63879670
CAACGCA15450.030.2635065
CTTATAC1600.029.8722481
AACGCAG15750.029.6870546
ACGAGAC1650.028.96481522
AGAGTAC16600.028.37036511
CGCAGAG17100.027.5429278
CAGAGTA17450.026.9904910
GCAGAGT17600.026.5665429
ATGCCGT1705.456968E-1226.5038744
CTCGTAT1705.456968E-1226.35866239
Produced by FastQC (version 0.11.5)