FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1918587
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG103880.5414401327643729No Hit
GATTATTACTGTGCAACGTGGGATGACAGCCTGAATGGTGTGGTCTTCGG88000.4586708864388219No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAAGACCAC78150.4073310201726583No Hit
GACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAAGACCACACCATTCAGG69590.36271485212815474No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC67200.3502577678260095No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAACCACCCTCAGCGTCTGGGACCC64970.33863463059011656No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT57010.2971457640440595No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTC55510.28932751029794324No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAACCACCCTCAGCGTCT53310.2778607381369727No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG50860.2650909236849827No Hit
GTGCTGACTCAACCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC50480.26311029940263325No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG49120.2560217493394879No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC47640.24830773897665312No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG46360.2416361624466339No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAACCAC45610.23772703557357577No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG43820.22839725276987702No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC42930.22375842221384798No Hit
CCCCTGAGGGCCGCTGATCATTGTTGTAGATGAGGAGTTTGGGGGCCGTT42740.2227681100726733No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG41390.2157316817011686No Hit
GTATTAGCTCCGATATTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT41260.21505409970983855No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG40090.20895586178786785No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA39860.20775706288013002No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT39240.20452551799840196No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA38730.2018673117247224No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT37780.19691575101884878No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC36850.19206843369625667No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT36340.18941022742257713No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC36190.18862840204796552No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA36080.188055063439917No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA35400.18451078840834426No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA34460.17961134939411139No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC34330.17893376740278133No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG33200.1730440162473737No Hit
GTTCTGGTACCAGTTTAAAGTATTAGCTCCGATATTGGAGCTGCTTCCAG32750.17069854012353883No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAACGTGGGATGACAGCCTGAATGGTGTGGTCTTCGGCGGAGGGAC32470.1692391327575971No Hit
ACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAAGACCACACCATTCAGGC32160.1676233603167331No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC31800.1657469794176652No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT31380.16355786836875263No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC31000.16157724408640317No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG29070.15151775759973354No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT28870.15047532376691805No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC28800.15011047192543264No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28730.14974562008394718No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT28640.14927652485918022No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA28600.14906803809261712No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC28300.14750438734339386No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA28210.14703529211862687No Hit
GTCATCCCACGTTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCC27380.14270919171244253No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT27060.14104129757993772No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAAGACCACAC26880.14010310713040378No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAA26720.13926916006415135No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG26610.13869582145610285No Hit
GTTTGGGGGCCGTTCCTGGGAGGTTCTGGTACCAGTTTAAAGTATTAGCT26460.1379139960814912No Hit
CTCTTGTTCTGGAAGCAGCTCCAATATCGGAGCTAATACTTTAAACTGGTACCAGAACCTCCCAGGAACGGCCCC26460.1379139960814912No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26300.13708004901523882No Hit
GTTTAAAGTATTAGCTCCGATATTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGA25950.13525578980781167No Hit
GTTGTAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTTCTGGTACCAGT25940.1352036681161709No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG25930.13515154642453014No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC25820.1345782078164816No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC25560.13322304383382144No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC25550.13317092214218068No Hit
GTGTGGTCTTCGGCGGAGGGACCGAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC25180.131242419551472No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC24940.1299914989520934No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG24880.12967876880224874No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC24870.12962664711060795No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT24850.1295224037273264No Hit
CCATTCAGGCTGTCATCCCACGTTGCACAGTAATAATCAGCCTCATCCTC24630.12837572651122936No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG24430.12733329267841384No Hit
GAGCTAATACTTTAAACTGGTACCAGAACCTCCCAGGAACGGCCCCCAAA24230.12629085884559835No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTCGGTC24140.12582176362083136No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA24000.12509205993786052No Hit
GAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACGCTGAGGGTGG23750.12378901764684115No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC23480.12238173197254021No Hit
GTCTGTGCTGACTCAACCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAG23090.12034898599854998No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC23070.12024474261526842No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAATATCGGAGCTAATACT22950.11961928231557911No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC22930.11951503893229756No Hit
GTCAGGGACCCCTGAGGGCCGCTGATCATTGTTGTAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTTCTGGTA22890.11930655216573448No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC22690.11826411833291896No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT22330.11638773743385106No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC22210.11576227713416176No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22200.11571015544252097No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA22060.11498045175955013No Hit
AGACCACACCATTCAGGCTGTCATCCCACGTTGCACAGTAATAATCAGCC21930.11430286976822006No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTACAACAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG21920.11425074807657927No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAATATCGGAGCTAATACTTTAAA21760.11341680101032688No Hit
TTGTTGTAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTTCTGGTACCA21690.11305194916884144No Hit
ATTCAGGCTGTCATCCCACGTTGCACAGTAATAATCAGCCTCATCCTCAG21630.1127392190189968No Hit
GACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC21440.11174890687782207No Hit
GGCCCAGTCTGTGCTGACTCAACCACCCTCAGCGTCTGGGACCCCCGGGC21420.11164466349454051No Hit
ACTTTAAACTGGTACCAGAACCTCCCAGGAACGGCCCCCAAACTCCTCAT21170.11034162120352113No Hit
GCCCAGGACCCTGCACAGTGAGTGAGGAGGGTGAGGAGGAGAGGGAAGCTGGCCATGCTGGAGATTGTCCTGAGT21140.11018525612859881No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT21070.10982040428711337No Hit
GTACCAGAACCTCCCAGGAACGGCCCCCAAACTCCTCATCTACAACAATGATCAGCGGCCCTCAGGGGTCCCTGA20840.10862160537937555No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG20830.10856948368773477No Hit
AGTCAGCACAGACTGGGCCCAGGACCCTGCACAGTGAGTGAGGAGGGTGAGGAGGAGAGGGAAGCTGGCCATG20810.10846524030445322No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC20750.10815251015460858No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT20750.10815251015460858No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT20340.10601552079733678No Hit
ATATTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGT20280.10570279064749215No Hit
AGTCTGTGCTGACTCAACCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG20200.10528581711436594No Hit
ATCTACAACAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC19700.10267973253232716No Hit
GATCATTGTTGTAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTTCTGG19510.10168942039115246No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAACCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC19490.1015851770078709No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG19450.1013766902413078No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG19350.10085547332490004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)