FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1918587
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAACGTGGGATGACAGCCTGAATGGTGTGGTCTTCGG81300.4237493530395025No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAACCACCCTCAGCGTCTGGGACCC70990.37001188895786324No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC70200.36589427531824203No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAAGACCAC66680.34754743986068914No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG56700.2955299916031955No Hit
GACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAAGACCACACCATTCAGG56460.2942790710038169No Hit
GTGCTGACTCAACCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC55630.28995297059763253No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG53440.2785383201283027No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAACCACCCTCAGCGTCT53420.2784340767450212No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT53370.2781734682868173No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG49130.2560738710311286No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTC47900.2496629029593133No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAACCAC47380.24695257499399298No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG45720.23830037418162428No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC43770.22813664431167313No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA43020.22422751743861496No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG42620.22214264977298398No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC41790.21781654936679964No Hit
CCCCTGAGGGCCGCTGATCATTGTTGTAGATGAGGAGTTTGGGGGCCGTT41470.2161486552342948No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG40380.2104673908454503No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA38420.2002515392838584No Hit
GTATTAGCTCCGATATTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT38220.19920910545104287No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT37990.19801030654330504No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAACGTGGGATGACAGCCTGAATGGTGTGGTCTTCGGCGGAGGGAC37250.19415330136188766No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC37240.1941011796702469No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG37220.19399693628696535No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA36630.19092175648015963No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA34400.17929861924426674No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT32920.171584608881432No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA32830.17111551365666502No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32820.17106339196502424No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT32770.17080278350682038No Hit
GTTCTGGTACCAGTTTAAAGTATTAGCTCCGATATTGGAGCTGCTTCCAG32730.17059429674025728No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT32550.16965610629072334No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT31960.1665809264839176No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC31810.16579910110930596No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG31480.16407908528516038No Hit
CTCTTGTTCTGGAAGCAGCTCCAATATCGGAGCTAATACTTTAAACTGGTACCAGAACCTCCCAGGAACGGCCCC31160.16241119115265557No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC31040.16178573085296627No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30750.1602742017953838No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC30440.15865842935451976No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC29620.1543844506399762No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA29150.15193473113285977No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT28800.15011047192543264No Hit
GTTTGGGGGCCGTTCCTGGGAGGTTCTGGTACCAGTTTAAAGTATTAGCT28350.14776499580159774No Hit
GAGCTAATACTTTAAACTGGTACCAGAACCTCCCAGGAACGGCCCCCAAA27910.14547164136940363No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG27720.1444813292282289No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG27710.14442920753658814No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAATATCGGAGCTAATACT27300.14229221817931634No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG27290.14224009648767555No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAA27160.1415625144963455No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC27140.14145827111306394No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27060.14104129757993772No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC26750.13942552513907372No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG26670.1390085516059475No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA26500.1381224828480543No Hit
GTCTGTGCTGACTCAACCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAG26430.1377576310065689No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25850.13473457289140395No Hit
ACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAAGACCACACCATTCAGGC25540.1331188004505399No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24990.13025210741029727No Hit
GTGTGGTCTTCGGCGGAGGGACCGAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC24960.13009574233537494No Hit
GTACCAGAACCTCCCAGGAACGGCCCCCAAACTCCTCATCTACAACAATGATCAGCGGCCCTCAGGGGTCCCTGA24910.12983513387717108No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC24900.1297830121855303No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC24890.12973089049388953No Hit
GTTGTAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTTCTGGTACCAGT24450.1274375360616954No Hit
ACTTTAAACTGGTACCAGAACCTCCCAGGAACGGCCCCCAAACTCCTCAT24280.1265514673038022No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG24150.12587388531247215No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC24090.1255611551626275No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTACAACAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG23970.12493569486293819No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAATATCGGAGCTAATACTTTAAA23960.12488357317129743No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT23830.12420599117996733No Hit
GTTTAAAGTATTAGCTCCGATATTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGA23700.12352840918863725No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC23530.12264234043074407No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC22950.11961928231557911No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22750.11857684848276362No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAAGACCACAC22700.11831624002455975No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTCGGTC22580.11769077972487044No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC22530.11743017126666656No Hit
ATCTACAACAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC22500.11727380619174423No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT22250.11597076390072486No Hit
GGCCCAGTCTGTGCTGACTCAACCACCCTCAGCGTCTGGGACCCCCGGGC22160.11550166867595787No Hit
GAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACGCTGAGGGTGG22090.11513681683447245No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG21690.11305194916884144No Hit
GCCCCCAAACTCCTCATCTACAACAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT21610.11263497563571524No Hit
ATATTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGT21280.11091495981156965No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA21220.110602229661725No Hit
GTCAGGGACCCCTGAGGGCCGCTGATCATTGTTGTAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTTCTGGTA21200.11049798627844347No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC21160.11028949951188037No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA21060.10976828259547261No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAACCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC21030.10961191752055029No Hit
TTGTTGTAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTTCTGGTACCA20420.10643249433046299No Hit
AGTCTGTGCTGACTCAACCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG20410.10638037263882222No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT20210.10533793880600671No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAAGAC20180.10518157373108439No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC19910.10377428805678345No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC19790.10314882775709416No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT19680.10257548914904563No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC19600.10215851561591943No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19220.10017789133356997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC4400.051.4493752
GTGGTAT4600.048.5222171
GGTATCA13400.044.6707231
GTATCAA33200.039.0135271
CACTAAT8000.035.47482370
TATCAAC36850.034.7503932
TCAACGC40300.031.7638844
ATCAACG40450.031.645273
CAACGCA40850.031.4180815
AACGCAG41600.030.8516486
CGCAGAG43250.029.439618
AGAGTAC43750.029.02974311
GCAGAGT45150.028.1259299
ACGCAGA46850.027.2502387
ATAGCGG550.00911798226.68546163
CGTGCAA1356.2191248E-926.57532757
TTAACGG1307.2595867E-926.19266935
TAGTACT659.801605E-425.9810144
CAGAGTA49550.025.76334810
AGTACGG47500.025.10235613
Produced by FastQC (version 0.11.5)