FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775115
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27750.35801139185798236No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20220.26086451687814066No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA18640.2404804448372177No Hit
GAGTTACATAACGGGGACCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT18310.23622301206917684No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17980.23196557930113595No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA17970.2318365661869529No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17820.22990136947420706No Hit
GGCCAAAAGTGGTCCCCGTTATGTAACTCTGTTGACAGTAGTAAGTTGCA17650.22770814653309512No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17300.22319268753668808No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16880.21777413674099974No Hit
ATGTAACTCTGTTGACAGTAGTAAGTTGCAAGATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAAATCTGTC16760.21622597937080304No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15530.20035736632628706No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGTACTTATTTAAATTGGTATCAGCAGAAA13980.1803603336279133No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13950.17997329428536413No Hit
GTAGTAAGTTGCAAGATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA13550.17481276971804183No Hit
CACCATCACCAGTCTGCAACCTGAAGATCTTGCAACTTACTACTGTCAAC13250.17094237629255016No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13190.1701682976074518No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13050.168362114008889No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13030.16810408778052288No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGTA12990.16758803532379066No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12970.16733000909542453No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT12970.16733000909542453No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC12590.1624275107564684No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12560.16204047141391922No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT12470.1608793533862717No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12430.1603633009295395No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG12320.15894415667352585No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12020.15507376324803418No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11930.15391264522038667No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11840.15275152719273913No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11820.15249350096437303No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11680.15068731736581023No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11300.14578481902685408No Hit
ACTTACTACTGTCAACAGAGTTACATAACGGGGACCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA11100.14320455674319293No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11090.14307554362900987No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC10860.1401082420027996No Hit
GATTTCACTCTCACCATCACCAGTCTGCAACCTGAAGATCTTGCAACTTA10790.1392051502035182No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10690.13791501906168763No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10610.13688291414822318No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10490.13533475677802648No Hit
GTACTAATGCTCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGA10210.1317223895809009No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT10020.12927114041142282No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA9970.12862607484050753No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT9670.12475568141501585No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9590.12372357650155137No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9580.12359456338736832No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC9540.1230785109306361No Hit
ATAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCAATTGCAGATCCA9530.12294949781645305No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9310.12011120930442579No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTT9260.1194661437335105No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9060.11688588144984939No Hit
GGTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG9040.11662785522148326No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA9010.1162408158789341No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8990.11598278965056798No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8980.11585377653638491No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8840.11404759293782213No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8840.11404759293782213No Hit
CAATAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCAATTGCAGATC8820.11378956670945603No Hit
ATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA8800.1135315404810899No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC8800.1135315404810899No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8540.11017719951233043No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG8390.10824200279958458No Hit
ACATAACGGGGACCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA8310.10720989788612012No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT8300.10708088477193707No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8250.10643581920102178No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8090.10437160937409287No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7960.10269443888971314No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7940.10243641266134702No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7880.1016623339762487No Hit
CCTTTAGTACAATAGCTGTCCTCGCACAATAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCAATTGCAGAT7850.10127529463369951No Hit
GTCTGCAACCTGAAGATCTTGCAACTTACTACTGTCAACAGAGTTACATAACGGGGACCACTTTTGGCCAGGGGA7770.10024318972023506No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAA200.007563574751.390810
CTACGTC200.007563574751.39083
GTGGTAT1800.049.5481761
TACGCAT451.7459788E-646.42658249
GTATCAA16850.046.2117921
TATCAAC18650.041.7085652
GCTTGTA1100.040.5394171
TCAACGC19650.039.4090544
CAACGCA19650.039.2321475
ATCAACG19800.039.1054573
AACGCAG20050.038.7912376
GAACTAT451.0356735E-438.113981
CGGGATT451.04001476E-438.08691417
AGAGTAC21450.036.25706511
CGCAGAG21600.036.166238
GCAGAGT21950.035.5895469
ACGCATC501.7551183E-434.8450750
ACGCAGA23850.032.610667
GAGTACG21600.032.20063412
AGTACGG21400.032.02125513
Produced by FastQC (version 0.11.5)